The Response Of Brachypodium Distachyon In Low Calcium Stress And Its Glutamate Receptor Family Gene Identification And Function Analysis

Ca²⁺ is one of the most important nutrient elements in plants. But the calcium that plants can use has declined in the soil and water year by year, as well as the animals. After Brachypodium distachyon genome was sequenced in 2011, the new mode of gramineous plant was begen to be study depthly. Brachypodium distachyon Bd21 was treated with low calcium to analyze the phenotype and absorption of elements, and the microstructure change. At the same time, changes of gene expression were analyzed in the stress of low calcium. Expression profile chip analysis showed AtGLR2.8 gene which was annoted in Arabidopsis expression rose observably. Glutamate （Glu） is one of primary neurotransmitters for neurons communication mediated by glutamate receptor （GLRs） on the membrane. Glutamate receptors in mammals are divided into two families: ionic glutamate receptor （iGLuRs） and metabolism of glutamate receptors （mGLuRs）. Physiological, pharmacology and genetic evidences indicate that.Arabidopsis and Rice have the similar signal transduction mechanism as that in mammals. In this study,19 glutamate receptor genes were found in Brachypodium distachyon genome through bioinformatics analysis. These receptors had conservative amino acid binding sites and membrane structure domain, and were similar with the ionic glutamate receptor （iGLuRs） and AtGLRs in Arabidopsis thaliana. Glutamate receptors in Brachypodium distachyon （BdGLRs） can be divided into three classes and their expression showed issue specificity. Glu could induce Ca²⁺ influx in the root cells of Brachypodium distachyon and modified its root architecture. So there is the mechanism that response to Glu in Brachypodium distachyon.