The Design Of Cultivating Device Using High-cell Density Culture Of Spirulina And Optimizing The Producing Technology

A new type of open reactor was designed, a airlift loop photobioreactor, and because of the factors affecting the cell growth of Spirulina platenis (PSP),the operating parameters were optimized. At the same time, the experiment researched that the PSP cell growth in autophototrophic cultivation and mixotrophic cultures in bioreactor with in unsterilization..Firstly, two bioreactors were designed. One is 5L plate bioreactor and the other is 5L circle column bioreactor, and some parameters were preliminarily optimized. The changes of light intensity with cell density and optical length in the liquid cultures of PSP were investigated and based on the experimental data the light attenuation in the culture process were described in two photobioreactor. The final dry cell densities of PSP were got in the designed bioreactor higher than in the cocking incubator, and the plate photobioreactor was better than circle column one ,so it is indicated that air-lift loop photobioreactors were more suitable for the cultivation of microaglae.Then, a 20L-airIift loop photobioreactor was devised according to the studying of 5L photobioreactor and its parameters was reformed. The light attenuation in the culture process were described in the 20L photobioreactor by the same method. In addition to, some parameters like light intensity , gas circulation velocity and inoculum volume were optimized ,so the final result is that light intensity is 5000lx,and gas circulation velocity is 100V/h and the volume of inoculum is 80%. Finally, the experiment proved satisfactorily that the processing of cultivated PSP in the mixotrophic cultivation was not contaminated in the open air-lift loop photobioreactor, and preliminarily researched that the influences of organic carbon source (glucose) on the cellular growth of PSP.