Construction Of Coexpression Vector For Hepatitis B Virus DNA Vaccine And Immune Response Induced By It

There are 350~400 million HBV carriers in the world, and 90 million of them will haveliver disease in the future. About 1~2 million patients will die each year.There are about 170 million HBV carriers and 17 million chronic hepatitis patients in China,it is severe for us. Each year almost 500,000 die of liver cirrhosis and liver cancer.It is a very useful method to prevent Hepatitis B ,injecting vaccine. And it issafe,effective , relaible method since years. Injecting the vaccine is recommend in thecountries HBV is epidemic heavly. In China, vaccination of HBV is a part of vaccinationsystem of the country.Our experiment is planning to construct expression vector of gene of HBsAg andGM-CSF. GM-CSF is an immunologic adjuvant to improve the effection of HBV DNAvaccine. We clone the Parvovirus IRES gene into plasmid pVAXâ… MCS. And gene ofHBsAg and GM-CSF is cloned IRES up and downstream MCS. So, we construct the doubleexpression vector pHIG. Using the double expressive plasid pHIG to immunizing BaLB/Cmice (the experiment group);using pVAX injecting BaLB/C mice (the control group).Theywere immunized after the 2ed,4th weeks.HBsAb was expressed after 1 week in the experiment group, not in the control group.The double expressive plasid of HBsAg and GM-CSF can evok the mice specific humoralimmunity and improve the immunal effection one time.