| The bacterial community composition of nine samples retrieved from Pacific Arctic surficial sediment was investigated by PCR-DGGE (Denaturing gradient gel electrophoresis) methods. Bacterial and Archaeal l6S rDNA clone library of two sediment samples were also created to analyze phylogenetic composition and diversity.Using PCR-DGGE technique, the sequences of the V3 region of 16S rRNA gene retrieved from nine surficial sediment were separated. Examination of 16S rDNA sequences obtained from DGGE bands showed that bacterial phylogenetic composition of surficial sediments in the Pacific Arctic was diverse. 16S rDNA sequences corresponding to 51 excised bands from nine sediment samples were analyzed and fell into seven lineages of the domain bacteria:α-,β-,γ-,δ-proteobacteria; Cytophaga-Flexibacter-Bacteroides (CFB) group; Actinobacteria and Acidobacteria . The subclass of the Proteobacteria, especiallyγ-Proteobacteria predominated in this study. In nine sediment samples, the composition and distribution of bands separated from DGGE fingerprint were different. Five shallow-sea samples(S11,C19,P11,R15,P13) and two deep-sea samples(S26,B78) had more DGGE bands than the other two deep-sea samples(P27,S16).A 16S ribosomal DNA clone library of bacteria from sediment P13 was established. The results showed that 84 clones fell into eight phylogenetic categories:α-,β-,γ-,δ-proteobacteria; Actinobacteria; Acdiobacteria; Bacteroides and Verrucomicrobia. Sequences related to bacteria ofγ-proteobacteria predominated in this sample. 47 clones were related to the gamma subclass of the class Proteobacteria, 8 clones belonged to theδ-Proteobacteria and Acdiobacteria, respectively. 6 clones belonged to theα-Proteobacteria, 1 clone belonged to theβ-Proteobacteria,Verrucomicrobia and Bacteroides, respectively. The results of the 16S rDNA clone library constructed from...
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