Study On The Purification Of Mouse Embryonic Stem Cell And Its Differentiation To Neuron

Embryonic stem (ES) cells derived from the inner cell mass of the preimplantation embryo are capable of self-renewal. When grafted into a host embryo, they are capable of differentiating along all major embryonic lineages. ES cells can also differentiate into a variety of cell types in vitro. A significant problem is how to gain pure ES cells.We established a method of separating murine embryonic fibroblast from embryonic stem cells.ES cells were induced to differentiate into neuron cells.A simple method has been established to separate murine embryonic fibroblasts (MEF) from embryonic stem cells ( ES cells). By comparing the different adhesion rate of MEF and ES cells to find out the opitimum time for MEF removing. The result shows that 1.5h and 0.5% gelatin concentration is the opitimum condition for MEF removing. Furthermore, the ES cells which have been purified have the same plating efficiency and the ablity of three germ layers differentiation as the unpurified ES cells. After differential adhesion there is strong ALP activity in ES cells. All the results show that the purified ES cells are still in the state of undifferentiation and maintain the pluripotent.Es cells differentiated into neurocyte. First, to find out the suitable culture solution. Second, inoculate different density cells to cell culture plates. Third, to look over the effect of inducing-time and blood serum. At last,we differentiated ES cells to neuron cell.