Genetic Variation Of The Mitten Crabs Populations In Mainland Of China Inferred From Complete Mitochondrial Cytochrome B Sequences

Mitten crabs, which have the habit of procreant migration, are special taxa in Grapsiodea. This thesis including three chapters, makes a study of the genetic variation of the mitten crabs populations inferred from Complete Mitochondrial Cytochrome b Sequences.1. Introduce of the geographic distribution and the genetic diversity of the mitten crabs in Mainland of China.2. Seventy-three individuals of the mitten crabs from Yalujiang River, Liaohe River, Yangtse River, Oujiang River, Mingjiang River, Jiulongjiang River and Nanliujiang River in Chinese mainland were selected. Complete mitochondrial Cyt b gene sequences were sequenced, sixty-eight variable sites were determined and twenty-nine haplotypes were identified. The distances, based on the Kimura 2-parameter method with MEGA 2.0 software, indicated the genetic distances between the north groups and the south groups was higher than those within each groups. The phylognetic trees reconstructed by multiple methods (NJ, ML and MP) supported the similar topology. Minimum spanning network protracted with ARLEQUIN 2.0 software was consistent with phylogentic trees. And three possible presumes were put forward for the formation of E. j. hepuensis haplotypes in the northern basins based on haplotype diversity (h) and nucleotide diversity (Ï€) calculated with the molecular variation analysis software AMOVA and DnaSP.3. 50 individuals of the mitten crabs from Nanliujiang River in Chinese mainland were selected to detect whether there is haplotype of E. j. hepuensis with PCR/RFLP and DNA aequenced. From sequence analysis of mtDNA, thetwo haplotypes identified as E. j. sinensis, one was identical with a haplotype from...