The Construction And Assay Of Inducible Bax And Bcl2 Transgenic Cells Of Hypericum Chinese Linn

Production of secondary metabolites with clinically importance in plants by cell cultures has been one of the most extensively explored areas in recent years owing to the enormous commercial value of those compounds and the scarcity of the plants in the world. Application of plant cell culture for the production of usful secondary metabolites, however, is still limited due to the low yield of the desired compounds. The biosynthesis of secondary metabolites is widely believed to be the results of defense responses of plants to biotic and/or abiotic stresses. Therefore, the activation of the defense reactions of plant cells has been considered to be an effective strategy for improving the productivity of the secondary metabolites. Bax is a death-promoting member of the Bcl2 family in mammalians. It has been reported that overexpressing of Bax in tobacco and Arabidopsis plants induces defense reactions. We, therefore, hypothesize that expressing Bax in medical plant cells might activate the biosynthetic pathways of secondary metabolite and enhance its production. In order to verify this hypothesis, we develop the mouse Bax and Bcl2 transgenic Hypericum Chinese Linn suspension cells. The main results are summarized as follows:...