Study On The Nuclear Basic Proteins And Acid Phosphatase During Spermiogenesis In Some Species Of Decapoda Crustacea

During spermiogenesis in animals, spermatids change into spermatozoa. Their morphosis changes greatly. There is also a replacement of basic proteins which associate with DNA in the nucleus. The basic proteins' replacement is very important in maintaining the stability of genetic material and performing fertilization of sperm. Therefore, nuclear basic proteins play a critical role in the spermiogenesis, fertilization, and other reproduction functions.In the thesis, the distribution change of basic proteins and acid phosphatase was observed during the spermiogenesis of several species of Decapoda Crustacean, and the biochemistry character of the histones of Fenneropenaeus chinensis was also analyzed preliminarily, to research the replacement character of the basic proteins during spermiogenesis in Decapoda. The results were those:The changes of distribution of basic proteins, and the distributions of the basic proteins in the sperms of F. chinensis, Eriocheir sinensis, Macrobrachium nipponense and Procambarus clarkii during spermiogenesis were observed by TEM and ammoniacal silver reaction (ASR) in this paper. The results showed: (1) In the stage of early and middle spermatid stage, the basic proteind were producted in the endoplasmic reticulum, and kept in the endoplasmic reticulum vesicles. In the stage of late spermatid, the endoplasmic reticulum vesicles fused together to form an acrosomal vesicle. Meanwhile, the nuclear envelope became incomplete. The basic proteins migrated to the acrosomal vesicle from the nucleus. An acrosomal vesicle with compact basic proteins also was observed in the late stage of spermatid of Pro. clarkii. (2) There were basic proteins in the acrosomal complex of mature sperms of the four species, but the location of basic proteins had species-specificity. The basic proteins were also found in the sperm nucleus of F. chinensis and M. nipponense, but not in the sperm nucleus of E. sinensis and Pro. clarkii. It could be presumed that there was species-specificity in the transfering and replacement of the nuclear basic proteins binding DNA during sperm formation in Decapoda Crustacean.