| The protein analysis and immunoassay play a very important role in life sciences. It is widely used in molecular biology, biochemistry and clinical diagnosis. The disadvantage of the routine methods urged the researchers to find the new analysis methods with high sensitivity, simple operation and universal applicability.In this dissertation, based on the interaction between the nanoparticles and the proteins, Resonance Light Scattering (RLS) assay of proteins by using Cr (OH) 3 nanoparticles as probe and the conditions of Fe(OH)3 nanoparticle chemiluminescence (CL) detection for immunoassay.Under some conditions, Cr(OH)3 nanoparticles and protein can interact by electrostatic forces and result in aggregation of the Cr(OH)3 nanoparticles on the protein suface. The formed aggregation and the electric dipole-dipole interaction between neighboring particles will cause strong RLS, the enhanced RLS intensity is well proportional to the concentration of protein, and thus a simple, sensitive method of protein determination is established. The enhanced RLS intensity is well proportional to the concentration of bovine serum albumin (BSA) in the range of 0.08-1.6 μg/mL, and the detection limit (3σ) is 18.6 ng /mL. The RLS method exhibits lower variation in response signal for the same weight of BSA, human serum albumin (HSA) and γ-globulin (γ-G) and has been successfully applied to the determination of the total protein in human serum samples. The result is coincident with that of the Coomassie Bright Blue (CBB) method.Additionally, the conditions of chemiluminescence (CL) detection of Fe(OH)3 nanoparticles for immunoassay were investigated, which are the basis of further chemiluminescence immunoassay.
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