Cloning And Sequence Analysis Of A Gene Encoding For Light-harvesting ChlorophyⅡA/B-Protein And It's Promoter Of Gracilaria

A gene encoding for PSII light-harvesting protein and its'promoter is cloned, sequenced and analyzed from the red alga Gracilaria lemaneiformis from Qingdao.The length of the gene is 684bp, There is no intron in this gene. The molecular weight of the protein is 25kD.The protein has four transmembrane helices,six Chl a-binding residues , two Chl b-binding residues, three conserved motifs,three putative phosphorylation.There are some motifs required for light regulated in the promoter.By aligning the Light-harvesring protein's nucleotide acid sepuence of four species of Gracilaria, the molecular systematic relationship of them were surveyed. The similarities of nucleotide acid sequences of the light-harvesting protein among the Gtenuistipitata varliui , the Gracilaria lemaneiformis came from Qingdao and its'pigment mutants , the Gracilaria lemaneiformis came from South Africa are between 0 to 0.07.The similarity of nucleotide acid sequence of the light-harvesting protein of Gracilaria lemaneiformis came from Venezuela with the homlogous nucleotide acid sequence of them are between 0.087 to 0.092. We conclude the nucleotide acid sequence of this protein can be used for molecular phylogenetic analysis among different species and at higher taxonomic levels.