Cloning And Function Analysis Of Ats1A Gene Promoter And Ats2bB Gene Promoter From Arabidopsis Thaliana

Resently,great achievements have been maken in the study of transgenic plants asbioreaction producing recombinant proteins.It has becoming a new developmental trend inplant transgenic engineering. Making use of hight active and tissue specific promoter toincreace the quantity of the exogene expression in the specific developmental stage andspecific tissue or organs is a key point.Rubisco is the most abundant protein in the chloroplast in plants. The quantity of Rubiscois about 50% of that of the total soluable proteins in leafs. ats1A gene and ats2B gene are twoof Rubisco small unite genes in the cell nucleus of Arabidopsis thaliana .The study of theregular elements ,the promoter activation and tissue specificity of the two genes will find anew way of increacing the quantity of the exogene specific expression.Furthermore makinguse of the chloroplast transit peptide to make the product of the exogene expression in thechloroplast is another way to increacing the quantity of the exogene expression. in the presentpapers, ats1A gene Promoter(including the chloroplast transit peptide) and ats2B genePromoter(including the chloroplast transit peptide)was cloned by PCR. The sequences of thetwo promoters were analyzed. Ats1A gene Promoter and the ?-glucuronidase gene were fusedto construct p18T1a-GUS expression vector and the function of ats1A gene promoter wasanalyzed by transitory expression in the tabacco. The main results are as follows:1. The 5'flanking sequence of ats1A gene and ats2B gene including the promoter region andthe coding region of chloroplast transit peptide in Arabidopsis thaliana was cloned by PCR2. ats1A gene promoter and ats2B gene promoter was analyzed by the plant cis-actingelement datebase.3. Ats1A gene Promoter and the -glucuronidase gene were fused to constructp18T1a-GUS expression vector.4. p18T1a-GUS expression vector was transformed into tabacco by bombardment.Histochemically analysis show that GUS expressed in leafs and stems of tobacco.this resultshow ats1A gene promoter is a good candidate promoter in the study of transgenic plant asbioreaction.