A Study On The Related Genes Of Early Embryonic Development Of Bisexual Artemia From China

Brine shrimp(Artemia) is widely distributed in inner land salt lake and coasting saltworks, it belongs to Crustacea, Branchiopoda, Anostacea, Artemidae, Artemia. On the Artemia from China, little attentions have been paid to its morphology; developmental biology; ecology; resources biology; genetics up to date. But, little studies on the related genes of early embryonic development of bisexual Artemia from China were investigated, more thorough studies need us to finish. mRNA differential display was established by Liang and Pardee in 1992 for the purpose of displaying the mRNA differences between two tissues. Therefore, in the study, the related genes of early embryonic development of bisexual Artemia from Shan Xi in China have been studied by mRNA Differential Display. RNA samples in six development phases were reversed with three anchored primers and then amplified with twenty six random primers. After running on 10% non-denaturing PAGE gel and stained by Silver Nitrate, the differential display DNA bands were obtained. 211 DNA fragments were isolated. 20 DNA fragments among these which sizes were 260～800bp in length. After cloning and sequencing, these fragments can be used as ESTs as contrast with GeneBank dbEST database. 4 fragments with high similarity to many sequences in the existing database were obtained, named ed1-ed5. The results show that ed1 is most probably the fragment of glycine-rich protein. Glycine-rich protein involved in the salt stress response. It is very useful to Artemia because of its salty circumstance. ed2 might be the fragment of reverse transcriptase. ed3 is almost the fragment of short tandem repeats. ed4 is probable the fragment of catalase, and it could protect organism from damage.