Expression Of Gonadotropin Receptor MRNA And Culture In Vitro Of The Porcine Oocytes

In this paper, porcine ovaries collected from slaughters were used to obtain oocytes. Factors affecting oocyte IVC, such as paraffin oil and size of follicle were investigated. Expression of gonadotropin receptor mRNA in granulas in different size of follicles, different quality and maturation phase in vitro of oocytes were also studied by RT-PCR. The results were as follows:1. The maturation, clevage and Morula development rate of group without paraffin oil was higher than other group with paraffin oil, but there are not significant difference (P>0.05).2. Oocytes from different size of follicles had different development ability. Oocytes collected from large (φ>6mm) and medium( φ3-6mm) follicles had a higher maturation rate than oocytes from small(φ<3mm)follicles(P<0.05); Oocytes collected from medium( φ3-6mm) follicles had a higher cleverage rate than oocytes from small(φ<3mm) and large(φ>6mm) follicles P<0.05).3. The FSHR mRNA in granulas of COCs from different size of follicular had different ability of expression, the relative abundance of FSHR mRNA from large(φ>6mm) follicles had lower than medium(φ3-6mm) and small(φ<3mm) follicles P<0.05).4. FSHR mRNA abundance was affected by quality grade of COCs, the relative abundance of FSHR mRNA was significantly higher in quality grade A COCs than in quality grade C COCs (P<0.01) , there are significantly different between in quality grade A and B and between in quality grade B and C (P<0.05).5. Gonadotropin receptors mRNA abundance in granulas of COCs was affected by time of maturation, the relative abundance of gonadotropin receptors had significant difference between Oh and else time during maturation (P<0.05). There was a trend towards decreased FSHRmRNA abundance, and a trend towards increased LHRmRNA abundance during maturation in vitro.