Research On Homology Of Mannose Receptor Gene Between Sperm And Macrophage In Mouse

Objective:The aim of this investigation is to study the homology of mannose receptorgene between sperm and macrophage in mouse.Method:Total RNA was extracted from mouse peritoneal macrophage and a454bp-sequence of cDNA coded for mannose recognition domain in macrophageMR was obstained by RT-PCR.The fragment of cDNA was cloned into the pGEM-Teasy vector and sequenced.The sequence was proved to be correct according to thedatabase from the Genbank.The sense and antisense templets were made bydigestion of the plasmid pGEM-T easy MR with restriction enzymes Salâ… and Ncoâ… .The probe of MR with digoxingen-labeled of sense and antisense RNAfrommouse was constructed from the templets by transcription in vito with T7 and SP6RNA polymerase,then MR mRNA expression was examined with nonradioactive insitu hybridizaton in the mouse testis. Hybridization signal was visualized with thecolor substrates NBT/BCIP.Result:1. The sequence of macrophage MR cDNA fregment inserted in plamid is right by sequencing.2. The probes of MR with digoxingen-labeled of sense and antisense RNA from mouse were constructed from the templets by transcription in vito with T7 and SP6 RNA polymerase .3. In situ hybridzation analysis showed that hybridization signals can not be detected in cytoplasm of spermatogenic cells in all stages seminiferous tubule in testis.Conclusion:1. The probes of MR with digoxingen-labeled of sense and antisense RNA from mouse were constructed from the templets by transcription in vito with T7 and SP6 RNA polymerase .2. There was not the homology of mannose receptor gene between sperm and macrophage in mouse.