The Effect Of Two Anthocyanin Synthesis Enzymes And Three Different Promoters On The Plant Color

The color of leaves and flowers is a very important characteristic in plants. It is determined the value of the commerce. The color of leaves and flowers is determined by the kinds of four different pigments and their relationship, such as chlorophyll, anthocyanin, and carotenoids. In plants, chlorophyll, anthocyanin biosynthesis pathways and enzymes involved in these pathways were characterized. Up to date, many structural and regulatory genes controlling chlorophyll and anthocyanin were cloned. Comparatively, the study about important enzymes involved in the carotenoids biosynthetic pathways is very little. The basic strategy of flower color genes engineering include inhibiting the gene expression of the key enzymes, and transferring the regulator genes or the new exogenous genes into the target plants. But the study about leaf color genes engineering is almost vacancy. These may because that leaf is the most important organ of photosynthesis, the change of leaf color may result in abnormal growth and development. Otherwise, the relationship of anthocyanin and chlorophyll is not clear. So, in this paper, we study the effects of CHSA (chalcone synthaseA) and F3'5'H (flavonoid3′,5′-hydroxylase) and three different promoters(CaMV35S, PNZIP, AP3) on the color of leaf and flower of tobacco, and the relationship between anthocyanin and chlorophyll. The main results are as follows: 1. Based on the sequences of known CHSA and F3'5'H genes of Petunia hybrida, we designed the specific primes, two DNA fragments were amplified from flower of Petunia hybrida by PCR and RT-PCR techniques. Sequence analysis showed that the sequences of F3'5'H fragment coincidence with the published cDNA sequence of F3'5'H genes in GenBank, but the CHSA fragment is shorter about 500bp than reported CHSA gene. Further sequencing the full length of CHSA fragment, we find that the absent 500bp fragment exist in an intron.