The Influence Of A Maize Reguolator Gene Lc And A Melanin Gene On Flower Colour Of Transgenic Petunias And Tobaccos

The primers were designed according to the sequence of Brassica napus' (rapes') petal-specific promoter (GeneBank No. BD082011), and the promoter was amplified by PCR which was named XY355. The XY355 promoter was connected with maize's anthocyanin regulatory gene Lc, and with a modified melanin gene respectively to construct expression vectors pXY60 and pYC26.The expression vectors pYC26, pXY60, and pQT56 (the expression vector provided by our laboratory was containing CaMV35 promoter and Lc gene) were introduced to the Agrobacterium tumefaciences LBA4404.Then the leaf discs of petunias and tobaccos were transformed by Agrobacterium tumefaciences LBA4404.Some regenerated plants had been chosed at random and checked by PCR. Among these paints, there were 61 positive tobaccos including 23 plants of pYC26, 20 plants of pXY60, and 18 plants of pQT56, and there were 31 positive petunias including 15 plants of pYC26 and 16 plants of pXY60.Transgenic plants' flowers color had been observed. The negative control petunia lines (transformed by pBI121) had purple flower tubes and white.corollas. 8 plants of 16 pXY60 PCR-positive petunias had light purple corollas. Most pYC26 positive petunias had not evident alteration of flower color. However, one of pYC26 PCR-positive petunias had changed its flower color caused by a alteration of the flower shape.The negative control petunia lines (transformed by pBI121) had pink petals. 10 plants of the 20 pXY60 PCR-positive tobaccos had red petals and anther filaments. 15 plants of 18 pQT56 PCR-positive tobaccos had intense red petals and anther filaments. As for the tobaccos transformed by pYC2, no obvious changes in flower color had been observed.