Development Of Cloned Embryos Reconstructed With Nuclei Of Cumulus Cell From Self-or Different Individual Donors In Mouse

In the current thesis, the mouse cloning technique was established firstly. The nuclei from somatic cells (cumulus cells, CCs) taken from adult mice were introduced into enucleated oocytes B6D2F1 strain and the reconstructed embryos were activated by SrCl₂ and cultured with CZB medium for development in vitro. Secondly, we investigated the effect of different sources of CCs from self- or different individual donor (SID or DID) on embryonic development. The cloned embryos in the two groups shown no potency at various developmental stages (p>0.05), while their developmental rate at the 4-c (82.9%, 80.8% vs 95.7%), morula (75.2%, 70.4% vs 92.8%) and blastocyst (31.8%, 25.6% vs 72.5%) stages were significantly lower than that of the parthenogenetic controls(p<0.05). Finally, 77 of 1-c embryos and 37 of blastocysts from SID group, or 68 of 1-c embryos and 28 of blastocysts from DID were transferred into the foster oviduct and uterus., respectively, but none developed beyond 11 days post coitum. These results indicated that whether the: donor cell and the recipient cytoplast came from the same individual or not did not affect the rate of embryo development in vitro, but the effect on full development still need further investigation.