The RAPD Analysis On Genetic Diversity Of Hibernation Groups Of Rhinologhus Ferrumequinum

In order to have a sight into genetic diversity and relative relation of Rhinolophusferrumequinum, Randomly Amplified Polymorphic DNA(RAPD) analysis was applied to thetwo hibernated groups carried from Ji an in Jilin Province. Electrophoretic bands andexperimental data were used to calculate the average genetic similarity and genetic distancebetween two arbitrary individuals, and SPSS11.0 was applied to constitute the phylogenetictree. Twenty 10-mer primers(S131-S140,S2001-S2010;Sangon;Canada) were screened andsixteen were selected to amplify genomic DNA from twelve samples representing the twopopulations. A total of 104 RAPD loci were obtained among the two populations, and themaximum frequency of polymorphism was accounted 11.11 percent, the minimum wasaccounted 100.00 percent, the average was accounted 69.76 percent. The average geneticsimilarity and genetic distance between two arbitrary individuals were 0.9 and 0.1respectively. The results indicated that the degree of genetic similarity among individuals of the twopopulations was very high while the level of genetic diversity in the population was lowaccordingly. Furthermore pairwise comparisons and Hard-Weinberg balance were foundaccording to the data of the research. This situation should be owed to a rather frequency ofinbreeding among the two populations which we studied only has forty or fifty Rhinolophusferrumequinum, can't copulate randomly between individuals. Finally ,this situation causedpairwise comparisons and Hard-Weinberg balance. It is necessary for a species' adaptationand evolutionary potential to keep genetic variety at a relative level. Genetic diversity in thepopulation must not be lost furthermore and gene exchange among individuals should beenhanced as much as possible to avoid extinction of Rhinolophus ferrumequinum. At thesame time, close breeding should be strictly avoided, too. Protected policy would beestablished in order to avoid the further lost of genetic diversity according to the backgroundof genetic diversity and their habitation. That means genetic diversity in Rhinolophusferrumequinum can be sustained, and on the base the populations' adaptive capacity andevolutionary potential should be promoted. In this way, Rhinolophus ferrumequinum will beprotected more successfully.