DNA Damage Level Of Longevity And Non-longevity Families And Relevant Factors In Uygur Nationality In Xinjiang

Objective: This paper is to study the background levels of DNA strand breaks and the correlative factors in Uygur nationality in Xinjiang. Method: By way of epidemiology, the questionnaire was conducted, including such aspects as life-style, diet habits, smoking and alcohol drinking history, heredity history, reproductive history and case history, etc. After getting 108 subjects' permission, physical examination of each person was made, including height, weight, blood pressure and pulse. Meanwhile The blood samples were collected to evaluate DNA damage . in lymphocytes by using comet assay. Different statistical methods were employed, such as non-parametric test, t-test, correlation and regression analysis, covariance analysis, Crosstabes, Logistic regression and so on. Results: DNA damage frequencies between longevity families and non-longevity families are 14.33% and 21.93% , and they are statistically significant (P<0.01). In addition, the different grades of DNA damage are statistically significant, too (P<0. 01); DNA damage frequency is positively correlated with the age and will increase with the growing age. And its averages in different age groups (ages 20-39, 40-59 and 60-79) are 11. 18%, 21.63% and 23.17%, which are statistically significant(P<0. 01); the age was used as covariance to make covariance analysis, and it has been found that the remedy mean of DNA damage in the two groupswere still statistically significant (P<0.01). DNA damage frequencies of male and female were 17.98% and 17.19%, the difference was not statistically significant (P>0. 05). Logistic regression analysis found that low DNA damage Frequency as well as frequent bare feet, fruit taking, Hetian tea drinking constitute were the correlation factors of longevity. Conclusion: The non-longevity families have higher DNA damage Frequency than longevity ones and the difference is statistically significant. Excluding the factors of age, sex, smoking and alcohol drinking, etc, the mean of DNA damage Frequency in the two groups is still statistically significant . According to DNA damage and repair theory of aging, the life-span in the two groups may be related to the differences of DNA damage owing to different family backgrounds. Moreover, it has close relation with people' s life-style and diet habits.