Study On The Mechanism Of Glucose Induce The Development Block Of Mouse Embryos

At present, there is a common view that the glucose has an inhibitory effect on the embryo development in the earlier period but a facilitate effect in the later. However, there is no final conclusion about the mechanism of the earlier embryo inhibition. In this article, we carried a study on the mechanism of glucose induce the development block of mouse embryos with the mCZB for basal medium and Km mouse for study objects by adding glucose and antioxidant (GSH). In the experiment which tests the effect of adding glucose at different time to the mouse embryo development in vitro, the rate of blastocysts of the mCZB group, the group of from 1 to 4 day, the group of from 2 to 4day, the group of from 3 to 4 day and the 4 day group is 50.0 % ,55.3 % ,23.8 % ,55.3 % and 69.4 % .respectively. In the experiment of the effect of adding glucose in different stages on the mouse embryo development in vitro, the rate of blastocysts of the mCZB group , the 1st day group, the 2nd day group , the 3rd day group and the 4th day group is 40.5%, 12.8%,54.3% ,60% and!5.6%, respectively. In the experiment of the effect of exogenous GSH on the mouse embryo development in vitro, which was treated as glucose, the rate of blastocysts of the mCZB group, the glucose group and the glucose plus GSH group is 51.5 %, 14.8 % and 40.7 %, respectively .By analyzing the statistics and relevant data, we conclude: 1 the definite time that glucose inhibit the mouse embryo development in vitro is the second day; 2 the inhibition of glucose for the mouse embryo development in vitro can be overcome by GSH; 3 the reason of mouse in vitro developmental block may be the ROS that was produced by glucose oxidation.