Cloning Of The Stress-Inducible Transcription Factor DREB1A Gene From Arabidopsis Thaliana, Constraction Of Plant Expression Vector And Transforming Tobacco

The stress-inducible transcription factor DREB1A gene is amplified from Arabidopsis thaliana (Columbia ecotype) genome by polymerase chain reaction and cloned into pUC19 vector. Sequence analysis showed that the obtained gene is identical to the reported sequence. Subsequencly, the rd29A promoter was fused to the upstream of the DREB1A. Then the fusion gene, rd29A-pro : DREB1A, and the nos terminator of the pBI101.2 vector were constructed into plant expressed vector pPZP221.Tobacco plants were transformed with the expression vector by Agrobacterium-medlated transformation. Then, the gentamycin-resistant tobacco transformants were obtained. PCR detection demonstrated that the fusion gene has been integrated into tobacco genome.