The Relationship Between The Expression Of HSF1 And IL-1β,TNF-α MRNA Of Monocytes In Fever Rabbits

IntroductionHeat shock factor (HSF) is generally found in the eukaryotic cell, which is high conserved in the structure and function. HSFl is sensitive to all kinds stresses. HSFl transforms form inactivated monomer to activated trimerization that steps into the nucleolus and combines with the heat shock element ( HSE) , which lies in the upstream gene of protein 70 ( HSP70 ) , to promote HSP70 transcription and expression.Studies indicate that many inflammatory stimulators invade the body and activate monocyte to produce endogenous pydogen ( EP) , including interleukin-1 (IL-1) , tumor nectosis factor, which is the important key to induce fever.The promoter of IL-1 (3 gene has an integrate HSE sequence, and theTNF-agene near the promoter exists many incomplete HSE sequences. Consequently, it's deduced that activated HSFlont only conbines with the HSE sequence of HSP70, but also with IL-1 TNF-, and affects their transcriptions, there are few reports about whether HSFl regulates the temperature by affecting the expression of IL-l,TNF-during fever.Our study aim at the effects of HSFl expression on the level of the gene transcription of IL-1 ,TNF-a. We study the expression of HSFl and IL-13 , TNF-amRNA in monocyte and their relationship during different fever stages in rabbit fever model induced by LPS. This study is very critical to identify the mechanism of thermo-limitation.Methods1. Animol model preparation; male rabbits were used by their weight from 2.0kg to 3. Okg, provided by animal department. They were divided into sixgroups; the control group, LPS-feverish-80min, LPS- feverish-160min, LPS-fe-verish-240min, LPS- feverish-320min, UPS- feverish-400min group.2. IL-1,TNF-mRNA expression : RT-PCR assay3. HSF1 protein expression ; western blot ( protein immunoblotting)4. image analysis technique and data processingRESULTS1. The body temperature change: Intravenous injection of LPS (0.5 g/kg) produced the double-phase fever in rabbits. The body temperature increases to the first peak value at 60min-phase alter injecting LPS, and to the second peak value at 180min-phase.2. HSF1 expression in the monocyte: The total content of HSF1 (including monomer and trimerization) wasnt obviously change in all groups (p >0. 05) , but the content of HSF1 trimerization increased with the body temperature rise (p<0.01) .3. IL-1,KTNF-mRNA expression in the monocyte at the different phases during the fever: the expression of IL-1mRNA in the monocyte increased at 80min-phase, arrived at the peak at 160min-phase, decreased to the normal level at the 400min-phase . There were significant differences among all groups ( p<0. 01 ). TNF-amRNA production in the monocyte reached the peak value at 80min-phase, then decreased gradually and arrived at the normal level at 320min-phase. There were significant differences among all groups (p <0. 01)4. The relation between the content of HSF1 expression and the content of the IL-1 ,TNF-amRNA production in the monocyte; The relative analysis indicated that in the LPS-feverish rabbit model, the content of the trimerization of HSF1 manifested the negative correlation with the content of the IL-1, TNF-amRNA productions (r= -0.963, r= -0.970; p<0.01).CONCLUSIONS1. The content of the trimerization of HSF1 increased in the monocyte during the LPS-feverish rabbits.2. The content of the trimerization of HSF1 in the monocyte manifested the negative correlation with the content of the IL-1β,TNF-αmRNA productions.3. During the UPS-induced fever, it was possible that HSF1 limited the rise of the body temperature through repressing the gene expressions of the endogery pyrogen-IL-1β,TNF-α.