RAPD Analysis Of Genetic Differentiation Of Sabina Vulgaris Populations In Inner Mongolia

Genetic differentiation of Sabina vulgaris populations in Inner Mongolia was studied with Random Amplified Polymorphic DNA (RAPD) markers. After analyzed by Popgen32 Version1.31, the results were as follows.Through the amplification with 18 random primers, 124 repeatable loci in which 100 were polymorphic were detected. Percentage of polymorphic loci was 80.65%. Nei's index and Shannon's index were 0.286 and 0.428 respectively, and they were 0.232 and 0.347 within populations respectively. Nei's index and Shannon's index in each population were Mu Us sandland(0.258,0.386)＞Yinshan mountain(0.255,0.376)＞Hunshandake sandland(0.231,0.345)＞Helan mountain(0.184,0.281).The total gene diversity was 0.288. The gene diversity within populations was 0.233. The coefficient of genetic differentiation was 0.183. The genetic variation among populations was 18.3%, and within populations was 81.7%. The genetic diversities in 4 populations were similar, but the genetic differentiation also existed because of geographic isolation.The average genetic distance and Nei's genetic identity respectively were 0.0865 and 0.9181 among populations. By cluster analysis (UPGMA) based on unbiased Nei's genetic distance, the populations in Mu Us sandland and Yinshan mountain clustered at 1.928, then they clustered at 2.366 with population in Hunshandake sandland. At last, they clustered at 6.426 with Helan mountain. They were two clusters when the threshold was 2.4, which was correlative with habits.