| With the development of economy and increase of the population, the shortage of water is becoming serious in the worldwide . These directly result in enlarging of arid regions, which limit the agriculture production. Studying the mechanism of drought-resistant and enchancing capability of drought-resistant of crops is an important measure to solve the shortage of water, realize agriculture of saving water, guarantee the sustaining development of agriculture.Plants often encount many kinds of environmental stresses, The reactive oxygen species (ROS), including H2O2 primarily, O2- OH, 1O2 ,could be induced in plant cells in responsive to the stresses. Many phyisilogical and cell biological studies have indicates that hydrogen peroxide as second messager involved in ABA signaling in guard cells. Therefore, research on H2O2 signaling will be more important.So far, little is known the details of H2O2 signal transduction and its complicated relation with other signal molecular, knowledge about molecular mechanism of drought-resistant and its genetics, especially to expressions of main gene of drought-resistant and controls of upstream signal transduction. Mutant, a powerful tool of analyzing genetic process , has already been extensively used for research in plant growth, metabolism and signal transductionInfrared imaging techniques utilize every part of difference of infrared radiation of objects to obtain the details of the thermal images. With the breakthroughs of infrared thermography, upgrade of infrared imaging aparatus will be quicker; scope of application more wider continuously; precision and accuracy of measurement more accurate. Particularly application in the life science also continuous changes and improves.Firstly we maked sure method of isolating Arabidopsisis mutant by thermal imagings in many ways on trial, optimize conditions for isolation, and confirm the goodperiod of seedlings and fine density for isolation, select correct isolating pressure(drought and H2O2) that is fit for isolate mutant.Secondly we obtain an Ml population derived from 0.4%ethyl methanesulfonate (EMS)-mutagenized wild type Arabidopsis thaliana. With the tiny difference of temperature leaf between mutant and wild type for index sign,We isolate Ml seedlings by 8-10 days drought-stress and 5X 10-3mol/L H2O2-stress treatment by infrared thermography in a large scale.A great deal of roi ros doi and dos Arabidopsis mutant were isolated (the difference of leaf temperature between candidate mutant and wild type is very obvious,about 0.6-1.210). The future characterization and genetic analysis for candidate mutant were carried out and find that some candidate mutant (such as roi30 doil-1 doi0311131)have good phenotype by drought H2O2 ABA -stressed treatment.At the same time we also observe the development of candidate mutant at different growth stages carefully. Many modal difference between mutant and wild type at the same period were found, such as more rosette layering fatty and big in leaves, advancment or delay for the flower period, rosettes living in the main stem, shorten in figure, the amount of seed little,sterilization etc. These physiological and modal changes may reflect with maladjustment in expressions of some gene and confusion on their inner control,. We will futher study concrete and detailed function mechanism.
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