| Insects have powerful reproductive capacity, which based on its reproductive physiology. Studies of reproductive physiology can help us to further master the reproductive mechanisms and to control insects' fertility.In the present paper, to reveal law of gametogenesis and to explain the reproductive mechanisms of Oxya chinensis (Acrididae), thus design "biorational insecticide, some investigations are reported.Histochemical staining researches have been done on gametogenesis of Oxya chinensis, especial on oogenesis. Studies of reproductive physiology were promoted with the development of lectin immunohistochemical method. PNA binding glycoconjugates on Oxya chinensis gametal surface were detected with microscope after SABC labeling for better understanding of the formation and distribution of glycoconjugates during spermatogenesis and oogenesis. In ovary PNA binding glycoconjugates mainly appeared in early oocytes and later in follicular cells. In the cytoplasm of primodial germ cells and primary spermatocytes, a granule structure can be observed closed to the nucleus, and secondary spermatocytes, small and lots granule, closed to the membrane. And the distribution of PNA binding glycoconjugates changes during sperm maturation.The results indicate that (1) Carbohydrates of oocytes were first formed by themselves and then by follicular cells. (2) PNA binding glycoconjugates appeared in early spermatogenesis and expressed in primary and secondary spermatocytes. (3) The distribution of lectin receptor changed during spermiogenesis.In order to study the regulatory mechanism in gametogenesis of Oxya chinensis, the special expression of c-kit is investigated by immunohistochemical method and In situ hybridization. The results show that Kit positive protein granules first exist on the cellular membrane of differentiated spermatogonia, and then primary spermatocytes. In secondary spermatocytes, small and lots granule, closed to the membrane. And the distribution of Kit positive protein granules changes during sperm maturation. Theresults indicate that Kit protein plays a role during spermatogenesis and spermiogenesis.However, Kit protein doesn't exist in primary oogenesis, it exists only in the oocytes during third and fourth stage, and first exists on the membrane of follicular cells during the fourth stage, when proliferating. During the fifth and sixth stage, the columnar follicular cells begin to secret Kit protein and later decrease. The results indicate that Kit protein was first synthesized by oocytes and then by follicular cells and plays a role during oocytes growth.The mesodermal accessory glands are important secretory organ, which can secret many kinds of material (carbohydrates, proteins and lipids), the secretion of Orthoptera mesodermal accessory glands is the main composition of spermatic fluid, which can activate and nourish sperm, form spermatophore and promote females ovulation. Seminal vesicle is the place in which the united sperm stored. Structure and function of the mesodermal accessory glands and seminal vesicle in Oxya chinensis, were studied with gland specimen, paraffin sections and light microscopy method. The results are summarized as follows:The male accessory glands of Oxya chinensis includes fifteen pairs of tubules opening into the ejaculatory duct, eight of them are white and seven hyaline glands. The histological structure of male mesodermal accessory glands showed it consisted of four layers: simple epithelium, basal lamella, muscle layer and peritoneal membrane. White glands were classified into two main types on the basis of epithelial cells and secretion: acidity and particulate glands, and hyaline into three: basicity, mucus and mixture. The size, epithelial cells and secretion changed after eclosion.Seminal vesicle, scattered with united sperm, consisted of four layers: columnar cell and secretory cell, developed basal lamella and muscle layer, peritoneal membrane, the blind and the other part had some difference in histological structure.
|
PDF Full Text Request