Font Size: a A A

Study On Genetic Identification And Genetic Diversity Assessment For Germplasm In Auricularia

Posted on:2005-06-01Degree:MasterType:Thesis
Country:ChinaCandidate:Y L WenFull Text:PDF
GTID:2120360122493149Subject:Microbiology
Abstract/Summary:PDF Full Text Request
In this study, 29 strains from 3 Auricularia species were chosen for genetic identification and genetic diversity assessment by using methods such as morphological taxonomy, isozyme techniques , RAPD analysis, ERIC-PCR and ITS-RFLP. The results are as following:1 ERIC-PCR was for the first time applied in differentiating strains from edible fungi and proved to be more rapid and reliable than RAPD in Auricularia identification study. Taken the similarity coefficient as 75%, 29 strains of three Auricularia species were grouped into 6 and 9 clusters by RAPD and ERIC, respectively. ERIC-PCR clearly distinguished A. auricula from A. polytricha while RAPD failed. The analyses showed that RAPD was able to differentiate mainly at the species level, while ERIC is effective at the strain level. It was suggested that ERIC-PCR could substitute for RAPD in research related to the genetic identification and genetic diversity in Auricularia and other edible and medicinal fungi:2 To a certain extent, genetic differences among Auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively;3 In this study, genetic diversity in A. polytricha was higher than that in A. auricula:4 In this study, A. fuscosuccinea had a higher homology to A. auricula than to A. polytricha;5 Morphological characteristics validated the results from ERIC-PCR and provided a potential explanation for the higher similarity coefficient between A. auricular and A. fuscosuccinea;6 Southern hybridization was employed by choosing a strain from A. auricula as a probe which hybridized with A. auricula and A. fuscosuccinea except A. polytricha, further confirming the veracity of the results from ERIC-PCR;7 In this study, isozyme analysis could not cluster the 7 strains from three Auricularia species to different groups efficiently;8 2 strains from two Auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity. It was suggested to increase the categories of RE and strains tested to obtain the patterns with high polymorphism;9 No significant differences displayed using primers whether special for fungi or universal for eukaryotic biology when ITS region was tested by PCR amplification and the fragment was about 650bp.10 Auricularia spp. had great possibilities to be a sub-species from A. polytricha ;11 The method GuTC was proved to be able to obtain genome DNA from Auricularia strains conveniently;12 The relationship between morphological taxonomy and molecular biological taxonomy is supplementing and validating each other.
Keywords/Search Tags:Auricularia, germplasm, genetic differentiation, genetic diversity, ERIC-PCR, Auricularia spp
PDF Full Text Request
Related items