| Recent studies indicate that there is a remarkable similarity in early heart development pattern among Drosophila, vertebrate and human. Studies of genes related to heart development in Drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. To clone and identify new genes that control the heart development, by a way of chemical mutagen ,EMS, we have established 1,200 balanced-lethal lines on chromosome 2 and 3.With the screening the 330 stocks with immunochemical method using heart-specific antibody, Mab.No.3, we detected 60 lethal lines showing heart mutant phynotype. The mutant lines were crossed with deficiency lines on chromosome 2 and 3 respectively, and the genetic sites of 11 stocks have been determined. Of them four showed a different phenotype from the known genes that control the heart development. Based on the deletion region, the possible candidate genes of 20 stocks were searched in flybase. They mainly function as transcription factors, translation factor, kinase, signal molecules, and so on. These candidates affecting heart mutant phenotype are to be identified. At the same time, a mutant stock has been established with P element mutation.
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