Experimental Studies Of Self-organization Mechanism For CGC Formation In Process Of Bacterial Waving Growth

In this article, the experimental studies on bacterial waving growth from vegetative cell (VC) to cryptic growth cell (CGC) have been reported. With bacterial CGC as main subject, the tests had been done to elucidate mechanism of self-organization for macroscopic rhythmic structure. The dynamics of CGC forming was observed by special techniques of waving culture and microscopic culture; The differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning; Integrity of cell wall was examined under TEM; Outer membrane protein was analysed by SDS-PAGE and various substance and factors for CGC formation were determined. It showed that bacterial colonies formed via number increase, exhausting nutrient, releasing waste, and results in environment far-from-equilibrium. Meanwhile, the bacteria so quickly change themselves in structure and function that they can adapt the varying circumstance. It involves morphological filament, physiological low metabolism but active movement. It is material basis and around effects for self organization of CGC forming that the speed quite different between waste diffusion and CGC active movement. On the other hand, the bacteria release BRF continuously, which accelerates the CGC to blast into VC at certain site. This just reflects self-regulation. In this process, the bacteria metabolism become lower and lower, that is important for CGC form. The work not only disclosed the mechanism of order structure formed in wave growth but also discovered the essence that the bacteria keep survive along with human-being owing to highly adaptive ability.