| Adiantum reniforme L.var. sinense Y. X. Lin, which is the second grade national protection plant, is the special plant in Three Gorge of Yangtze River.China. At present, it faces to die out, so it is extremely necessary to research its protection biology.The experiment uses Adiantum reniforme L.var. sinense Y. X. Lin as material to understand its mating system through the research of ecology and genetics. The main contents include: 1) the investigation and analysis in ecology. There are five natural sample zones of Adiantum reniforme L.var. sinense Y. X. Lin investigated, and recorded 34 species. 10 dominant species were used to calculate the niche width of populations and the niche overlap interpopulations. Several adult individuals from each population at random were observed their morphologic characters, and then to analyse the distribution of the vertical biology yield. 2) Studies on the mating system of Adiantum reniforme L.var. sinense Y. X. Lin using Allzyome Marker. The experiment employ six enzymes: EST, MDH, FDH, GDH, SOD and ACP , using their polymtic locus to analyse the allele gene frequency, the number of the effective allele gene. Hardy-Weinberg equilibrium test, the frequency of expected and observed heterozygosity and Chi-Square(X2) test of fixation index. Finally, the character parameter of the mating system Adiantum reniformeL.var. sinense Y. X. Lin -outcrossing ratio was estimated indirectly. 3 ) Spatialautocorrelation study of Adiantum reniforme var.sinense.The results are followed:1) Adiantum reniforme L.var. sinense Y. X. Lin has three kinds of population structures:.1. Berchamia sinica +Broussone tia kazinoki-Iris tectorm + Arthroxon hi spidus+ Adiantum reniforme var. sinensis. 2. Berchamia sinica + Vitex negundo-Iristectorm+Selaginella sp.+Arthroxon hispidus+Adiantum reniforme var. sinensis. 3. Vitex negundo-Arthroxon hispidus+ Adiantum reni forme var. sinensis. the second population structure is the best environment but the third one is the worst in three population.2) the sexual reproduction organs in Adiantum reniforme L.var. sinense Y. X. Lin holds comparatively high ratio, the vertical distribution of biology yield increases with plant height. This correspond to its biology character.3) Niche width of Adiantum reniforme L.var. sinense Y. X. Lin is high comparatively. The index is 0.4496 and 0.6888 respectively. This demonstrated that the adaption ability of Adiantum reniforme L.var. sinense Y. X. Lin is very strong.4) The niche overlap, the value is 0.3515, between Adiantum reniforme L.var. sinense Y. X. Linand Iris tectorm is the biggest. It demonstrated that there is strong competition between two for nature resource.The value of the niche overlap with Cyrtomiian fortunei is secondly bigger, it is 0.3389.5) The allzyome variety of Adiantum reniforme L.var. sinense Y. X. Lin : there are 7 polymorphic loci in the 14 loci of 6 kinds of enzymes system tested. The meaning number of the allele gene is compatively low for each locus, the value is from 1.43-1.57, the meaning number is 1.51; the change of the allele frequency is big in different loci, and the value is close to the low side, the percent of polymorphic locus is low and the value is from 0.3571 to 0.5. the mean expected heterozygosity is variged from 0.177 to 0.284. The observed heterozygosity is from 0.194 to 0.284. It shows the genetic variety of Adiantum reniforme L.var. sinense Y. X. Lin is low comparatively.6) The outcrossing rate of Adiantum reniforme L.var. sinense Y. X. Lin is high, the value is from 0.000-3.500. The different locus outcrossing rate of Adiantum reniforme L.var sinese Y. X. Lin has great change in the same population, and the outcrossing rate difference of the same enzyme locus is also high in the different population. It demonstrates Adiantum reniforme L.var. sinense Y. X. Lin is a mixed mating system and the intergametophzyme outcrossing among perhaps plays a main role.7) The result of Spatial autocorrelation study showed: m... |
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