Study On Enzyme-Mannanse And Its Origination

With the development of people's live standard,much importance are attached to health food,how to explore these natural source,particularly of Amorphailsu.the key lies in finding high valued production,in this aspect,how to get high activity enzyme is the prerequisite.ObjectionSelecting,inducing demanded strain (high enzyme productive),originated from nature,short ferment cycle,high resistance to environment factors.Measuresbacteria culturing,separating,confirming,analysis of activity and properties of Enzyme-ManrianseResultsBrachybacterium sp A6 is high productive in generating Enzyme-Mannanse,particularly in Amorphailsu.pH7.0,temperature 36 C,Carbon content 2.5%,suitable for Brachybacterium sp A6.Activity and stability of Enzyme-Mannanse changed according to different cultural condition.ConclusionA systematic method for preparation of Enzyme-Mannanse is established,a high productive strain was got after seducing and selecting from nature,confirmed as Brachybacterium spA6Research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation,the experiment result shows the optimium condition is pH7.0,temperature 36C;Carbon content 2.5%,ventilation in abundence,agitation speed 200r/min. enzyme activity of Mannanse reached 340u/ml (250ml),a leader in today's china.Activity and stability of Enzyme-Mannanse changed according to variety of cultural condition. The optimum condition of enzymes activity is in pH6.0,temperature 55 C .Enzyme activity also changed according to different kind of Ion and Ion content,Mn2+ positive eflfect;Fe3+,Al3+,EDTA,Mg2+ negative effect.