Experimental Study On Effects Of Sound Wave On The Growth Of Chrysanthemum (Gerbera Jamesonii) Roots

Plant and environment are dependent on each other. Now the effect of environmental factors on the growth of plant is one of important researches in plant physiology and biological physics. The research on effect of sound wave, compared with the electromagnetic wave, light and heat, on the growth of plant was practiced lately. It has been found that sound wave could greatly influence the growth of plant. In this thesis, chrysanthemum seedlings were cultured and the effect of sound stimulation on their roots was explored. The main contents and results of research in this thesis are as followes:Firstly, chrysanthemum seedlings grew faster when treated under sound stimulation with a certain intensity (100dB) and frequency (1000 Hz) for 9 days, and each day for 60 min. The relative penetration of plasmalemma of the stimulated group had no obvious change and the content of soluble protein improved greatly, which indicated that the growth of chrysanthemum seedlings wasn't inhibited but accelerated under sound stimulation.Secondly, the contents of soluble sugar and protein and the activity of roots and α-amylase of chrysanthemum roots under sound stimulation were obviously higher than that of the control. There is a very close relationship between the content of sugar and protein and the growth of plant. And the activity of root can reflect the absorption capacity of seedlings. So the results indicated that the growth and metabolism of chrysanthemum under sound stimulation was strengthened.Thirdly, the change of physical states of plasmalemma was studied using plasmalemma vesicle purified by two-phase partition. The results showed that the fluorescent intensity of DPH decreased and the fluorescent intensity of MC540 increased under sound stimulation, which indicated that the vesicles got looser, the charge density of membrane surface and the plasmalemma hydrophobicity decreased but the membrane fluidity increased. The secondary structure of protein plays an important role in the maintenance of function. In this thesis, the effect of sound stimulation on amideⅠand Ⅱ of plasmalemma protein was studied using infrared spectrometer. Detected variation of amideⅠand Ⅱimplied that the secondary structure of plasmalemma protein changed under sound stimulation.Fourthly, the activity of plasmalemma H+-ATPase increased under sound stimulation.We found that the activity of plasmalemma H+-ATPase could change under different concentration of Ca2+, and it was inhibited by Ca2+ passage blocker (Verapamil) and protein kinase inhibitor (Staurosporine) but was increased by Ca2+ carrier (A23187). The results demonstrated that the activity of plasmalemma H+-ATPase under sound stimulation was probably regulated by phosphorylation-dephosphorylation process.