Detection Of The Polymorphic MSPⅠ Site At COL1A2 Gene For Coreans Yanbian Of China

To study the polymorphism and allele frequency MSPⅠ site at COL1A2 gene for koreans Yanbian ,and contrast to other area population ,and give important genetic information to understand the origin and moving and correlate disease at COL1A2 gene of the population and genetic structure and genetic background; and to provid part medical genetics data for creation of immortalization cell bank for koreans China.we extracted human genomic DNA using phenol/chloroform extracting; and detection polymorphism MSPⅠsite at COL1A2 gene by the PCR -RFLP technique. A 600bp fragment from human genomic DNA was amplied by the PCR -RFLP technique,The PCR product was partly sequenced with dynabeads-streptavidin solid ,to detect PCR product and enzyme cut product by agovid gel electrophoresis and observing result in infared meter gel aut made aerial image ,Direct reading genotypic and calculation genotypic frequency and gene frequency.In 120 coreans, homozygotes with no digestion MSPⅠsite are 89 and genotype frequency (P-P-) is 74.17% ; heterozygotes with digestion MSPⅠsite are 22 and genotype frequency (P+P-) is 18.33% ; homozygotes with digestion MSPⅠsite are 9 and (P+P+) is 7.5% . The frequency of P+ is 16.7% in 240 allele of COL1A2 gene 。The polymorphism site at COL1A2 gene is associated withcoreans Yanbian ;and distribute of the polymorphism MSPⅠ site at COL1A2 gene is different in coreans Yanbian and other areas, so the polymorphism MSPⅠsite of COL1A2 gene as genetic marker of koreans Yanbian .