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Study On The Possibility For Classification Of Hemiptera Insects By Using Esterase Isoenzymes,Digestive Enzymes And The Internal Microorganism

Posted on:2004-03-01Degree:MasterType:Thesis
Country:ChinaCandidate:J LiFull Text:PDF
GTID:2120360092495716Subject:Agricultural Entomology and Pest Control
Abstract/Summary:PDF Full Text Request
The availability for classification of Hemiptera insects by using the esterase isoenzynes, microorganisms and digestive enzymes characters has been studied. The results are as follows.1.There were differences in the electrophoretic pattern of the bugs. Differences of zymograms between families were more significant than those between genera. Although zymograms within genera were rather similar, all the species could be unequivocally distinguished from each other. The feasibility about using isoenzymes in systematics was also discussed.2.The microorganisms in the bugs' body were identified through bacterioscopy; isolation and culture.The result showed that microorganisms were bacterium. The flesh-eating bugs, Nabis stenoferus Hsiao and Deraeocoris punctulastus Fall, had identical colony character; plant-eating bugs, Dolycoris baccarus (Linuacus) and Nysius ericae (Schilling), also had the same ones. However the significant differences of colony exited between flesh-eating bugs and plant-eating bugs.Bacterium were found in ova; nymphs and adults of Dolycoris baccarus (Linuacus). All the bacterium in the bugs' body could decompose protein, but could not break down starch. The transparent rings formed by bacterium decomposing protein between flesh-eating bugs and plant-eating bugs were different. The former were wider than latter.3.The ratio of amylase to protease enzyme activies(A/P) was used as one of the criteria for identifying the feeding habits of bug.The A/P for plant-eating bugs was about 2.0-6.0, for the flesh-eating bugs was 0.3-0.8. The rate of plant-eating bugs was fairly higher than that of flesh-eating bugs.
Keywords/Search Tags:electrophresis, esterase isoenzymes, the internal microorganism of bugs, digestive enzymes active, bugs
PDF Full Text Request
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