Study On Physiological And Biochemical Characters In Cell Culture Of Camptotheca Acuminata Decsne

Camptothecin is a kind of anticancer medicine having good prospect of exploitation. But lacking of resources, production of camptothecin by cell culture may be a promising choice. In this paper, based on the young leaves of Camptotheca acuminata Decsne., callus were induced and screened, and the influences of light, hormones and several factors on the growth, physiological, biochemical characters of solid culture of Camptotheca acuminata Decsne. were primarily studied, the results were as follows: The optimal medium of callus induction and growth of Camptotheca acuminata Decsne. was B~ with the addition of 0.Smg/L 2.4-D. 1 .Omg/LNAA and 0.5 mg/LKT. In different light qualities, the cell growth circles were about 27 days. and the cell growth curve was like 搒? The green light accelerated callus抯 growth. while the blue light did the opposite effect. When Camptotheca acuminata Decsne. was cultured in different illuminative conditions, the contents of soluble protein, activities of peroxidase(POD). superoxide dismutase (SOD) and esterase all showed two peaks which were on the different days; The varieties of three enzymes?activity were all consistent with which C.acuminata抯 cell trowth rate in illumination was quicker than that of in dark. The electrophoresis analysis of protein抯 composition and isozymes showed: they all had changed in callus culture cycle and revealed the correlations between the callus growth and physiological and biochemical characters. In the solid culture, the inoculum quantity of 3g.FW/50m1 medium was better; the optimal concentration of Sucrose was 30g/L ; as the sources of Carbon. the effect of Glucose was better than that of Sucrose, and the medium with 30g/L Glucose was good to cell growth; the medium with addition of 0.1% Agarose increased the quantity of cell growth, but LH inhibited it.