The Separation And Purification Of Anti-Bacterial Peptide From The Skin Of Chinese Forest Frogs And The Studies Of Its Partial

Antibacteral peptide(ABP) existing in the biosphere with the biotic activity is a kind of little peptide and has the action of antibacteria commonly and even can resist some protozoa,virus and tumour cells. The Antibacteral peptide has become one of the hot spots of foreign and domestic scholars' investigavitions who gave more and more favors to it as its no ill effect. The Chinese forest frogs belongs to the amphibian,which have exposed skins,have develop a series of protective mechanism gradually so as to resist the harmful circumstance factors during the long process of nature selection. There are abundant bioactive substances,which can kill many kinds of microorganism.In order to verificated the antibacteria substances of Chinese forest frogs,the skins of fresh forest frogs were drawn out by methanol solution80% and filtered with sephadexG-100,sephadexG-50 and sephadexG-25.The substance with antibacteria action which had high purity was tested with Escherichia coli,Staphylococcus aureus,Bacillus pyocyaneus and Yeast by the antibacteria test to decide the minimal inhibitoryconcentration as 6.37umol.L-1,3.18umol.L-1 ,6.37umol.L-1,6.37umol.L-1 25.48umol.L-1. Only single band was observed in aquacare-SDS-PAGEwith the substance purificated from the forest frogs. The substance with a molecular weight of 6.28kDa has very strong cation character determined by the electrophoresis of diaphragm wafer of fiber of acetic acid. The substance with antibacteria action obtained from forest frog is made up of alanine,aminoacetic acid,leucine,Isoleucine,Proline,aminoglutaric acid,threonine,Serine,lysine. The substance with antibacteria action is a kind of poly peptide with a micromolecul.The background to discuss the value of the ABP used as a kind of drug was developed by the purification and evaluation of the skin of the Chinese forest frogs.