Constructing Porcine Myostation Gene Expressing Vector And Expressing It In E.coli

constructing porcine myostation gene expressing vector and expressing it in E. coliGrowth /differentiation Factor 8 (GDF-8), a member of transforming growth factor 3 superfamily, was a inhibitor of muscle growth and differentiation, which also was called Myostatin because the muscle weight increased 2-3 times after the gene was knocked out in transgene mouse. The double muscle beef, Belgian Blue was also caused by the natural deletion ofGDF-8.In order to study further natural functions of the porcine myostatin gene of which were obtain pGEM-3Z cloning vector in myostatin gene and to establish theory basis of molecular breeding of porcine, two pairs of upper, lower specific PCR primers which containing EcoR I and BamH I restriction enzyme sites was designed. The full-length and half-length myostatin cDNA was amplified to construct fusion-expressing vector and to transform E.coli BL-21cell expressing full and half length protein. We have achieved accurate expression that taken on disulfide bond(linage) gene.