THE PRIMARY STUDY ON THE CONSERVATION OF Malania Oleifera

Malania oleifera Chun et Lee , an evergreen broadleaves plant endemic to China , has beed ranked as the second-class protective spieces . Study showed it's endangered mechanism resulted form interior factors integrated with exterior factors . The author analysed the inherent endangered mechanism to search an effective measure to save this plant.(1)Flowering characteristics and pollen disper mechanism of Malania were studied in the field and the pollens were cultured under the laboratory . (2)Sands with different humidity were used to store seeds . Petsicides were used to disinfect the material and seeds of the storage . The author periodicaly sampled the stored seeds and analyse aetiological agents . (3)Seeds were stored at low temperature to study the effect of low temperature on seeds' dormancy. (4)The root nodule was chipped to examine under the microscope and it's nutrition content was tested . Additionally , seeds' generative reproduction were tried . Approaches of cutting and tissue culture were also studied to search an approach of increasing the area of Malania rapidly .The results are as follow : (1) The flower of Malania is hermaphrodite and the pollen dispersers are insets . The rate of the germination pollen cultured is very low . The pollens tube grow slowly and can be easily bent, which is possibly harm to pollination . The seed rate is very low . (2) The nodule is a part integrated to the root tissue . Nutrition contained in the nodules is similier to that of the root's . It possiblly resulted from microorganism . (3) The storing result is best with the humidity of the sand is 13% . Seeds lose its'life while the humidity of storing sand is too dry and easily become rot while too wet. (4 ) Seeds stored at 3 -5 癈 condiction delay it's germination . The mechanism that seeds delay it's germination under low temperature is still unknown . (5 ) The protective rate of seeds disinfected with petsicides is low . The cause may be that seeds do not mature enough . (6) Separated from rot seeds' tissue , 6 strains of pathogen and one eelworm have been purified . Artificicle inoculated test showed that Fusarium solani and the other two were the main fungus of the seeds . The symptom resulted form the eelworm is still unknown . ( 7 ) Test show seeding reproduction with low rate of germination and survive is still the chief ways at present. Stems of young tree used as explant cultured have adventitious sprout differentiation but don't have adventitious root . Embryoes used as explant cultured can grow into an integrated plant but it grow poorly . All these technic required a deeper study .