Optimization Of Condition For Producing Enzyme By Two Kinds Of Mixed Trichoderma Vride And Its Application On Corn Straw Degradation

Every year, about 700,000,000t of cornstalk is produced in the agricultural production of our country. It is the precious resource especially for our country whose arable area and forest are relatively inadequate. Taking advantage of complex microbial to degrade cornstalk can improve the rate of comprehensive utilization with the characteristics of sustainability, pro-environment and economical. So this subject is becoming the focus of researching.At present, the determination of the enzyme activities in CMC strains enzyme (carboxymethyl cellulose enzyme), FPA (filter paper cellulose enzyme) has been widely applied to straw microbial degradation research. However, the research of mixed bacteria producing enzyme technology optimization and the cornstalk degrading result after the inoculation of the mixed bacteria with high activity, especially the quantitative study of the relationship between the enzymes activity and degrading rate is rarely seen reported, as well as the study on the interactive quantitative model of the influencing factors in the actual process of fermentation and degradation.Hence, this research is to mixed cultivate trichoderma viride and trichoderma koningii, and works over the enzyme producing technology optimization of trichoderma viride and the relationship between it and the cornstalk degrading rate, hoping that it can provide theory basis for the microbiological deterioration of the cornstalk.Therefore main achievements are as follows:1.Trichoderma koningii mold production carboxymethyl cellulose enzyme and filter optimization study of enzyme conditions(1) Using multi-factor orthogonal experiment, the Trichoderma koningii hand in the mixed bacteria produce carboxymethyl cellulose enzyme and filter paper enzyme liquid medium, orthogonal test optimized by using object analysis results obtained for analysis, that the optimal scheme of media. Produce carboxymethyl cellulose enzyme its optimal solution for A3B2C4D1E4, namely carbon source for bran powder, concentration of lOg/L, nitrogen source for nitrogen source concentration of ammonium sulfate,0.11 g/L for 7.0, pH. Adopt a poor analysis, each factor to affect the size of the primary index> RB order:RE> RC> RA> RD to trichoderma viride, pH mixed bacteria produce sodium carboxy methyl cellulose enzyme, then is the biggest influence source of nitrogen, carbon sources, finally is nitrogen source types and carbon source concentration; The optimal filter paper enzymes in the scheme for A3B3C2D3E3, namely carbon source for bran powder, concentration of 10g/L, nitrogen source for yeast powder, nitrogen source concentration for Maurice/L for pH 6.0.(2)Choose the optimization of orthogonal test income to the Trichoderma koningii iquid medium, the mixed bacteria for single factor experiment, inspects different speed, temperature and quantity to produce carboxymethyl cellulose enzyme and the influence of filter paper enzyme enzyme live. The results show that, when the temperature is 30℃produced carboxymethyl cellulose enzyme when live and filter paper enzyme enzyme low temperature, highest enzyme live is low. Speed 150r/min in the cultivation of Cornwall, green under the condition of the mixed bacteria produce carboxymethyl cellulose enzyme and filter paper enzyme enzyme live, highest speed too high or low enzyme production live.(3) The optimization of orthogonal medium and obtain the optimum single factor experiment, green environment of training the mixed bacteria in Cornwall 30℃,150r/min conditions training and optimized medium enzyme production than the original medium enzyme production live living, carboxymethyl cellulose enzyme is original liquid medium enzyme production 1.5 times more live respectively, the enzyme 3014.32 U/L Filter paper cellulose enzyme is original liquid medium enzyme production of 1.5 times more respectively, the enzyme 2897.15 U/L.2. Trichoderma viride and trichoderma koningii corn stalk the mixed bacteria,the effect of degradation(1) The mixed bacteria chooses trichoderma koningii optimized the effect of corn straw medium degradation than trichoderma viride and trichoderma koningii in the original solution degradation of effect is good, because the culture medium for optimizing the mixed bacteria growth and trichoderma koningii produced a synergy effect, can make better use of corn straw, and promote the secretion of massively strains of carboxy methyl cellulose enzyme and filter paper enzymes and other enzyme, of corn straw have certain catalytic oxidation, the degradation of solution of corn straw respectively 38.6+0.13% rate, plus or minus 41.4 +0.12%,48+0.15%.(2) the mixed bacteria in optimizing trichoderma koningii medium secrete carboxymethyl cellulose enzyme and filter paper cellulose enzyme enzymes, high 3014.32U/L, by strains of enzyme degradation of corn straw live research on the effect of carboxy methyl cellulose enzyme, get enzyme activity of enzyme and filter paper on the correlation of degradation effects are certain, namely, the higher enzyme degradation liquid the better...