| The genes relative to Trehalose metabolism played significant roles on the growth, development, and anti-stress physiology of high plants. However, the functions on Classâ…¡family genes among them are still elusive. Combined with the trend on scientific research, we have selected and investigated the role of CbTPS5 up-regulated in low temperature by using the methods such as RACE, Western-blot, real-time PCR and transient expression of fluorescent fusion proteins in tobacco plants. The conclusions are shown as follows:1. Achieved the whole cDNA sequence of CbTPS5 and analyzed the AA/nucleotide sequence by Bioinformatics tools.2. Revealed an intimate relationship between CbTPS5, low temperature and high salinity. The mechanisms of CbTPS5 against above mentioned stresses are somewhat homogenous; this approves the overlaps of plant adaptation in different environments.3. CbTPS5 may regulate cell apoptosis and tissue death.4. CbTPS5 was localized on cell membrane. Integrating with the results of NaCl treatment, CbTPS5 may protect the channel protein and stabilize membrane structure against damage.In addition, we also expect to find the interactive protein with CbTPS5 by Coimmunaprecipitation to interpret its function on cell signaling.Our research has not only added the new member to the Cb Up-regulated Genes Library in the low temperature, but also elucidated the accurate function of TPS5 and Classâ…¡family genes, filled in the academic gaps.
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