Preliminary Functional Analysis Of MPS1 During Meiosis In Arabidopsis Thaliana

In Arabidopsis thaliana, MULTIPOLAR SPINDLE 1(MPS1) not only gets involved in the construction of spindle polarity during meiotic prophase I, but also plays an important role in homologous chromosome synapsis and the formation of DNA double-strand breaks during meiotic prophase I. The knockout mutant of MPS1 resulted in abnormal spindle organization, defective homologous chromosome synapsis and a total absence of chiasmata, which led to both male and female sterility. In this study, our investigation of MPS1 improved comprehension of the mechanism of meiosis. The construction of MPS1 with GUS fusion protein successfully complemented the phenotype of mps1 mutant plants. Histochemistry staining of MPS1: GUS fusion protein showed that MPS1 was expressed in various tissues in Arabidopsis. We also performed a yeast two-hybrid assay to explore the interaction between MPS1 and the other four proteins involved in DSB formation .The results showed that MPS1 may not directly participate in the assembly process of protein complexes during DNA double-strand break formation. Then we screened library by yeast two-hybrid and found that MPS1 interacted with C-terminal of a motor protein light chain. To investigate this gene , we carried out the protein sequence analysis , homology comparison and some preliminary functional analysis in Arabidopsis. These works lay the foundation for further understanding of how MPS1 participates in homologous chromosome synapsis, DSB formation and the molecular mechanism of spindle polarity.