Microbial Community Diversity In Seismic Fault Belt Spring In Xinjiang

The 10th spring of Urumq originates from Bogeda mountain of Tianshan mountain range, and the groundwater runoff in seismic fault belt. The spring discharges geochemistry elements which are migrated actively, such as sulfide and methane et al. In order to reveal the impact of hydrogeochemical changes to bacterial community in seismic fault belt spring water, the 10th spring water in Urumq was sampled periodically, we monitored the geochemistry elements, such as hydrogen, radon, methane, sulfide, methane, carbon dioxide, helium, fluorine and hydrarg, et al. The bacterial communities in the spring water were analyzed by cultivation and BIOLOG microplate analysis respectively. Microbial were collected by microporous membrane filtration and then total DNA were extracted by SDS-enzymatic disruption method. The V3 region of the 16S rDNA gene of those samples was analyzed by PCR-DGGE (Denaturing Gradient Gel Electrophoresis) and different bands were excised for sequencing. The DGGE fingerprints combined with hydrogeochamical parameters were analyzed by Canonical correspondence analysis(1) In total, 50 bacterial strains were isolated from spring water, and classified to phyla Proteobacteria (Alpha,Beta,Gamma), Bacteridetes and Firmicutes by 16S rDNA gene sequencing. This result suggested that spring water harbors a diversity of cultivable bacteria,(2) BIOLOG microplate analysis indicate that the concentration of sulfide in the spring water were significantly affected bacterial community structures, other parameters were inapparent(0.05 level,2tails). Microbial community in the spring presented kinetic changes and microbes in this environment showed preferential to certain carbon sources.(3) The DGGE fingerprints combined with hydrogeochamical parameters were analyzed by Canonical correspondence analysis (CCA). The result suggested that B16 (Uncultured bacterium) and epsilon proteobacterium were positive correlate to Fluorine fluctuation and clustered as group one and two, respectively; B1 (Flavobacterium), Pseudomonas aeruginosa, B10 (Uncultured bacterium) and Staphylococcus saprophyticus positive correlate to sulfide change; Thiomicrospira arctica, B3 (Flavobacterium) and Staphylococcus arlettae which positively correlated to hydrogen content. Bacterial communities in seismic fault belt spring water could response sensitively for hydrogeochemical parameter changes.