Functional Analysis Of MADS-box Gene Related To Flower Organ Development In Allium Sativum L.

Garlic is an important condiment.Due to the abortion of flower buds,normal seeds cannot be produced.Most of the existing cultivars can only be vegetatively propagated.This situation makes the study of garlic breeding limited to tissue culture,somatic asexual reproduction,and bulb induction.The inability to use hybridization means to breed new varieties has resulted in a large waste of garlic germplasm resources and cannot be fully utilized.Therefore,the recovery of garlic fertility will be beneficial to the change of its genetic traits and produce high-yield,spicy,virus-free green organic food.Therefore,in this study,the construction of five gene overexpression vectors was carried out using gateway technology.The function of the Arabidopsis thaliana was verified by the method of silk flowering,and their expression patterns were analyzed by real-time fluorescent quantitative PCR.To elucidate the function of the MADS-box gene related to the development of garlic flower organs.The main results of this study are as follows:1?Real-time quantitative PCR analysis of garlic flower organs showed that.The expression of AsAGL6 gene in carpels and petals was higher than that in other flower organs,and the expression in stamens was the lowest.The AsAG gene is expressed in the stamens and carpels at a higher level and the lowest in the sepals and torus.AsSEPl is the highest expressed in the receptacle and the lowest in the stamen.The AsSEP3 gene is expressed in higher amounts in petals,stamens and carpels.The AsPI gene has high expression only in petals and stamens.2?Real-time quantitative PCR analysis of flower development at different stages indicated that.The AsAGL6 gene was expressed most at the F3 stage.The AsAG gene is gradually increased in the development of the entire flower organ.The AsSEP1 gene has the highest expression in floral organ primordia.The AsSEP3 gene has the highest expression during the F3 period.The AsPIgene is most expressed when the floral organ develops into a complete flower.3?Overexpression vectors of AsPI,AsAG,AsAGL6,AsSEPl and AsSEP3 genes were constructed by Gateway technology.The wild type Arabidopsis thaliana was infested by the silk flower method.It is displayed by molecular detection such as PCR or RT-PCR.The foreign gene has been successfully introduced into the Arabidopsis genomic DNA.And expressed at the transcriptional level.Phenotypic observations of transgenic lines were shown.The AsAG gene has an effect of prematurely flowering wild-type Arabidopsis thaliana.Transgenic AsPIgene Arabidopsis petal dysplasia.The AsAGL6 gene has Arabidopsis stamen dysplasia,four stamens,and abnormal seed pod development.The transgenic AsSEPl gene Arabidopsis showed a phenotype of 5 petals and 4 stamens.The transgenic AsSEP3 gene Arabidopsis showed a phenotype of 5 petals and 5 stamens.