Genetic Linkage Mapping, Color Gene Location And QTL Analysis For Growth-related Traits In Bay Scallop Argopecten Irradians Irradians

Amplification fragment length polymorphisms (AFLP) and microsatellite were used for linkage mapping in F1 family in bay scallop, Argopecten irradians irradians. 2424 markers were detected from 55 AFLP primer combinations, among them, 392 (16.17%) are polymorphic. 121 markers segregated in female, 196 in male and 75 in both parents. Chi-square test indicated that 61 (15.56%) showed significant segregation distortion (P<0.05) from Mendelian ratio. Ten microsatellite primers selected from 35 published ones offered 5 female markers and 6 male markers.Separate linkage maps were constructed for female and male parents. The female framework map consisted of 115 markers (including 109 AFLP markers, 5 microsatellite markers and 1 shell color marker) in 16 linkage groups, spanning 338.8 cM with an average interval of 5.29 cM. 181 AFLP markers and 5 microsatellite markers combined the male framework map into 17 linkage groups, covering 780.3 cM with an average interval of 6.56 cM. The observed coverage was 65.82% in female map and 78.11% in male map. Through loci of SSR12 in both maps, F-G7 and M-G17 were probably the homologous linkage groups.Two markers, F1f335 and D8f420, were completely linked to the gene for orange shell, which we designated as Orange1, without any recombination in the progeny we sampled. The markers were amplified in the orange parent and all orange progeny, but absent in the white parent and all white progeny. The close linkage between F1f335, D8f420 and Orange1 was validated in inbred lines and two natural populations, and all...