Material Basis Identification And Pharmacological Mechanism Researching Of Fufang Xingxiangtuerfeng Against Cervicitis

Cervicitis is one of the common gynecological diseases in the clinic.It is characterized by a higher incidence and recurrence rate,a longer course of disease,and slower healing.It seriously disturbs the health and life of women,and is also a highrisk factor for infertility and cervical cancer.The current clinical diagnosis of cervicitis is mainly based on doctor's judgment,patient description and inflammatory index determination,but the pathogenesis of cervicitis is still unclear and it is worthy of indepth study.To clarify the material basis of the Fufang Xingxiangtuerfeng(FFXXTEF),this paper uses UHPLC-QTOF-MS technology to analyze and characterize the chemical composition of the FFXXTEF,and then establish the diagnostic ion and neutral loss filters according to different structural types of compounds.The strategy is used to identify the chemical composition of the compound.A total of 244 compounds were identified in the FFXXTEF,including 38 flavonoids,79 chlorogenic acids,65 lignans,29 sesquiterpenoids,and 33 other compounds.The study of the chemical components of the compound provides a basis for the study of the in vivo metabolism and medicinal substances of the FFXXTEF.To clarify the in vivo process and metabolic pathways of the FFXXTEF,this paper uses UHPLC-QTOF-MS to analyze rat plasma,urine,fecal bile,and establishes the "in vivo-in vitro,diagnostic ion filtration and neutral loss filtration " correlation analysis strategy,which is applied to the analysis and identification of metabolites in the body of compound apricot fragrant rabbit ear wind.A total of 122 metabolites,29 prototype components,and 93 related metabolites were detected in rats.24 were detected in plasma,which may be the active ingredient of the compound.76 were detected in urine,of which 19 metabolites were only detected in urine.60 were detected in feces,chlorogenic acids related metabolites were only detected in rat feces;29sesquiterpenoid-related metabolites were detected in bile,and only in bile.The main metabolic pathways of the FFXXTEF are hydrolysis,reduction,glucuronidation and sulfonation to clarify the dynamic process and metabolic pathways of the compound in vivo.To provide a basis for the in-depth study of the FFXXTEF anti-cervicitis drug discovery and mechanism of action.To further prove the clinical effect of FFXXTEF.This article first validates the rat model of cervicitis induced by phenol glue.The results show that the model of rat cervicitis replicated by phenol glue is simple,feasible and reproducible,and the model has a high success rate.After modeling,the organization maintains its original shape and biochemical characteristics.Secondly,the anti-cervicitis effect of compound Xingxiang rabbit ear wind was studied from the aspects of behavioral indicators,uterine index,structure of uterine histopathological and inflammatory factors.The results show that the FFXXTEF can significantly improve the pathological symptoms and vital signs of cervicitis rats;it can also reduce the bleeding,necrosis,inflammation and inflammatory cell infiltration of the uterine tissue,and it can also reduce the uterine index and serum L-The content of 1? and TNF-? play a role in treating cervicitis.The results show that the FFXXTEF can significantly improve the pathological symptoms and vital signs of cervicitis rats;it can reduce uterine tissue bleeding,necrosis,inflammation and inflammatory cell infiltration,and it can also reduce uterine index,content of L-1? and TNF-? in the serum,which plays a role in treating cervicitis.To clarify the pathogenesis of cervicitis caused by phenol glue and the anti-cervicitis mechanism of the FFXXTEF.This article establishes a rat model of cervicitis caused by phenol glue.And uses UHPLC-QTOF-MS technology to conduct metabonomics research on rat plasma and feces.In plasma metabolomics,63 endogenous differential metabolites were identified,mainly including lipids,amino acids,free fatty acid and other compounds.The main metabolic pathways include the biosynthesis of phenylalanine,tyrosine and tryptophan,arachidonic acid metabolism,glycerophospholipid,ether lipid metabolism,sphingolipid metabolism,secondary bile acid biosynthesis,etc.After the intervention of the FFXXTEF,49 of the potential biomarkers have a callback effect,which can respectively affect the biosynthesis of phenylalanine,tyrosine and tryptophan,arachidonic acid metabolism,sphingolipid metabolism,and glycerol Pathways such as phospholipid metabolism,primary bile acid metabolism,pantothenic acid and Co A biosynthesis have regulatory effects.A total of57 different endogenous metabolites have been identified in stool metabolomics,which mainly include vitamin B6 metabolism,?-linolenic acid metabolism,glycerophospholipid metabolism,sphingolipid metabolism,and amino acid metabolism.After the intervention of the FFXXTEF,53 of the potential biomarkers have a callback effect,which can respectively regulate vitamin B6 metabolism,?-linolenic acid metabolism,glycerophospholipid metabolism,sphingolipid metabolism and amino acid metabolism and other metabolic pathways.It shows that the FFXXTEF may play a role in the treatment of cervicitis by regulating the above-mentioned metabolic network.In addition,the common metabolic pathways found in rat plasma and feces are Phenylalanine,tyrosine and tryptophan biosynthesis,sphingolipid metabolism,aminoacyl t RNA biosynthesis,glycero-phospholipid metabolism,arachidonic acid metabolism,phenylalanine metabolism.In summary,this thesis clarifies the metabolic profile,physiological processs and metabolic pathways of the chemical markers of the FFXXTEF from the in vivo and in vitro perspectives.The effect of the compound on inflammatory factors will be studied from the perspective of in vivo pharmacodynamics Finally,UPLC-QTOF-MS metabolomics technology is used to clarify the active substance basis of the compound's multiple components and the therapeutic effect of multiple targets from an overall perspective.The results clarified the effective substances and mechanism of anticervicitis of the FFXXTEF.And laid a good foundation for people to understand the pathogenesis of cervicitis and the reasonable clinical use and secondary development of the FFXXTEF.