To Explore The Genetic Characteristics And Biological Mechanism Of Congenital Cold Syndrome Of CHD Based On TMT Technique

Objective: Through TMT(Tandem mass tag)proteomics technology,screen the differential proteins of coronary heart disease with congenital cold syndrome(CCS),analyze their biological functions,explore the biological mechanism of coronary heart disease syndrome,and establish markers for congenital syndrome Provide a basis for further research on the true connotation of the theory of "CCS",explore its pathogenesis,provide a basis for clinical diagnosis and treatment,and make Chinese medicine more precise.Analyze the differentially expressed proteins of two different nodes in the offspring of coronary heart disease with the characteristics of cold and cold,and whether the differentially expressed proteins are related to the formation of arteriosclerosis,explore the genetic characteristics of the CCS,and provide early clinical intervention Provide research basis for disease and screening susceptible people of coronary heart disease.Methods:Congenital cold syndrome of coronary heart disease and other different syndromes of coronary heart disease(choose four common syndromes of coronary heart disease that have an overlap with this syndrome-Qi deficiency and blood stasis syndrome,heart and kidney yang deficiency,heart and kidney yin deficiency,qi stagnation Blood stasis syndrome)and healthy people are used as the research objects,using TMT technology combined with liquid chromatography and tandem mass spectrometry(LC-MS/MS)technology to find the differential protein with specific expression,combined with bioinformatics technology,analyze the function and function of the differential protein The participating pathways,combined with PRM protein quantification technology,verify the differential protein,determine the reliability of the differential protein,and confirm the biological mechanism of this type of population susceptible to coronary heart disease.A longitudinal study will be carried out on people with the syndrome of obscured cold in coronary heart disease,and the offspring with the characteristics of obscured cold will be used as the research object,and the population without the above-mentioned syndrome characteristics and healthy people will be compared with each other.The protein is also used for comparison.Omic analysis,find the biological substances associated with the formation of arteriosclerosis,at the same time,combined with the PRM protein quantitative technology to verify the specific protein,and determine the experimental results.Results: 1.Screening differential proteins through TMT technology,and found that there are 5 differential proteins common to congenital fu-cold syndrome and healthy people,as well as the other four groups of syndrome types,which are immunoglobulin variable 4-69(Immunoglobulin lambda variable 4-69),Prenylcysteine oxidase(Prenylcysteine oxidase 1),fructose-bisphosphate aldolase A(Fructose-bisphosphate aldolase A),complement C3(Complement C3),soluble calcium-activated nucleotidase(Soluble calcium-activated)nucleotidase 1).2.The bio-information analysis of the differential protein found that the function of the differential protein between Fu Han syndrome and each group is mainly binding,catalytic activity,signal transduction and transport activities,etc.;differentially expressed proteins are mainly involved in cellular processes,biological regulation,and stress Important biological processes such as reaction,metabolic process,progress and positioning of immune effect.The pathways that the differential proteins of Fuhan syndrome participate in are mostly related to cell signal transduction or metabolism.3.Verified by PRM,the trends of the 4 differential proteins are consistent with the results of TMT quantitative protein screening.Among them,Fructose-bisphosphate aldolase,AImmunoglobulin lambda variable 4-69,and Prenylcysteine ??oxidase 1 are up-regulated in Fu Han syndrome;Complement C3 is down-regulated in Fu Han syndrome.4.The differential proteins of the offspring of coronary heart disease with Fu Han syndrome were screened by TMT technology and verified by PRM.The final 5 differential proteins showed the same trend,namely Reticulon-4 receptor-like 2,Desmocollin-1,Mannose-binding protein C,Serglycin,Monocyte differentiation antigen CD14.Among them,Desmocollin-1 is a differential protein that is up-regulated in the age of 14 years of Fuhan;Serglycin is a differential protein that is up-regulated in the age of 35 years of Fuhan;Mannose-binding protein C is a differential protein that is down-regulated in the age of 35 years of Fuhan;Reticulon-4 receptor-like 2.Monocyte differentiation antigen CD14 two proteins showed up-regulation at 14 years old and 35 years old.5.According to bio-information analysis,it is found that the differential protein functions of the two age nodes of the offspring are similar to those of the coronary heart disease syndrome.They are both involved in celluar process,metabolic process,and biological regulation.),biological functions such as regulation of biological process(regulation of biological process,positive regulation of biological process,negative regulation of biological process);molecular function analysis shows that there are mainly binding and catalytic activity;Cell component analysis shows that there are mainly cells,cell parts,organelles,extracellular regions,cell membranes,etc.;all participate in Cell adhesion molecules,Complement and coagulation cascades,PI3K-Akt signaling pathway these pathways.Conclusion:The genetic characteristics and biological mechanism of coronary heart disease syndrome may be related to Cell adhesion molecules,Complement and coagulation cascades,PI3K-Akt signaling pathway.Fructose-bisphosphate aldolase A,Immunoglobulin lambda variable 4-69,Prenylcysteine oxidase 1,Complement C3 may be the syndrome markers of coronary heart disease syndrome.The offspring of Fuhan found that the differential protein Mannose-binding protein C is closely related to Complement C3.It further confirms that the syndrome of obscured cold of coronary heart disease has a certain genetic tendency,provides a research basis for early clinical intervention of the disease,and screens the susceptible population of coronary heart disease,and provides ideas and targets for further research and treatment of the syndrome of obscured cold.