TCM Syndrome Classification, Syndrome Distribution And Differential Protein Related To Different Syndrome Types Of Refractory Epilepsy

Objective: From the point of view of evidence-based medicine to explore refractory epilepsy syndrome type,the characteristics of research methods,explore the intractable epilepsy type classification of syndrome,the syndrome factor distribution characteristics and laws;From the perspective of the differences in protein expression,explore the different refractory epilepsy syndrome,the molecular nature of this syndrome,to clarify intractable epilepsy of traditional Chinese medicine,to provide favorable scientific theory and experimental basis,to the new targets for developing new antiepileptic provides the theoretical foundation of new drug of TCM.Methods: Comprehensive the use of "literature induction analysis method,expert questionnaire,multicenter flow" to research the refractory epilepsy syndrome of TCM of the type of classification and element distribution characteristics;using immune histochemical method to protein variations associated with pathological changes of the hippocampus in patients with refractory epilepsy surgery for testing.Results:1.Through the literature review of TCM syndrome type classification and analysis,involving disease syndrome has a reputation for weights integral descending order followed by liver,god,heart,spleen,kidney,brain collaterals.The descending order of the integral descending order of the antipathic syndromes is phlegm,fire,air,blood,air,blood stasis,movement,closure,Yin and deficiency.The value of virtual syndrome is23.76%,and the real sex ratio is 69.41%,and the value of the virtual reality is 6.83%.2.Through expert questionnaire survey of refractory epilepsy syndrome type,stage attacks and attacks the type classification of syndrome and syndrome factor analysis,expert consensus-card type parts,involving disease syndrome factor 6 items,liver,kidney,spleen,god respectively,brain and heart;Involved in disease syndrome factor 10,respectively,phlegm,blood stasis,wind,and qi stagnation,closed,fire,qi deficiency,blood deficiency,Yin deficiency,jing kui,virtual syndrome factor weight of 20.84%,solid syndrome factor is 66.9%,the actual phase and syndrome factor weights 12.26%;The expert consensus-the phase of the onset of the study involved the position of the disease in the position of the disease.Involved in the disease of sex of weight integral sex syndrome factor is more than 5% of the disease are closed,the wind,phlegm,Yang deficiency,gas is not solid,wu Yang,virtual syndrome factor weight of 20.84%,solid syndrome factor weights is 33.50%,the weights of factors syndrome factor is 19.34%;The expert consensus-interphase study involved the liver,the heart,the spleen,and the kidney in relation to the weight of the disease in the position of the disease.Involved in disease of sex of integral right value is greater than 5% of the disease syndrome factor respectively,Yin deficiency,phlegm,blood deficiency,blood stasis,dampness,Yang deficiency,qi deficiency,and qi stagnation,blood heat,virtual syndrome factor weight of 40.83%,solid syndrome factor weights is 56.36%,the weights of factors syndrome factor is 2.81%.3.Through the clinical epidemiological investigation of TCM syndrome type classification and analysis,a weight integral is greater than 5% of disease syndrome factor mind,liver,kidney,meridian,heart,spleen,The weight of the weight is greater than 5% of the disease-sexual syndrome,which is active,closed,sputum,blood,Yin,Yang,and air.The value of virtual syndrome is 41.19%,and the value of real sex is44.92%,and the value of the virtual inclusion is 13.88%.4.Deficiency of grey value is greater than the empirical MCP-1;Each card type MCP-1grey value to compare differences between significant statistical significance(P<0.001);Deficiency syndrome of comparative differences between the two type certificate has no statistical significance(P>0.05),the empirical comparison between three syndrome types difference had no statistical significance(P>0.05);Two deficiency syndrome of spleen deficiency syndrome center has significant difference compared with the empirical three syndrome types statistical significance(P<0.01);Deficiency of spleen and kidney deficiency syndrome have significant difference compared with the empirical three syndrome types statistical significance(P <0.001).5.Each card type P-gp grey value to compare differences between significant statistical significance(P<0.01);Deficiency syndrome of comparative differences between the two type certificate has no statistical significance(P>0.05),the empirical wind phlegm closed orifices and change the phlegm heat syndrome between comparative differences had no statistical significance(P>0.05);Qi and blood stasis and phlegm wind in the empirical comparison between closed know-how and change the phlegm heat syndrome differences significant statistical significance(P<0.01),while with glove two deficiency syndrome and spleen kidney deficiency syndrome compared differences had no statistical significance(P>0.05);Glove two deficiency syndrome of deficiency syndrome and spleen kidney both deficiency syndrome compared with obsessed with the empirical wind phlegm closed the difference was statistically significant(P<0.05),and compared with the liver depression phlegm heat and qi and blood stasis has no statistically significant difference(P>0.05),in patients with qi and blood stasis in brain tissue of P-gp grey value of the minimum.Conclusion:1.Through the literature review of TCM syndrome type classification and analysis,treatment-resistant epilepsy deficiency syndrome types include: liver-kidney Yin deficiency,anaemia empty,liver kidney essence loss,spleen and kidney deficiency,spleen Yin deficiency,glove two virtual six card type.Empirical syndrome types include:in the autumn zephyrlily move,wind phlegm orifices closed,wind resistance of phlegm and blood stasis,phlegm and blood stasis resistance closed,phlegm fire the one god,nitrogen stasis,liver depression phlegm heat and blood stasis resistance brain collaterals,qi and blood stasis,a total of nine card type.The liver was the first in the disease.Phlegm is the first of the disease.2.Through expert questionnaire for treatment-resistant epilepsy syndrome stage,attack,attack the type classification of syndrome and syndrome factor analysis found that wind phlegm was the main syndrome types of refractory epilepsy closed orifices,nitrogen stasis syndrome and phlegm fire one god,the autumn zephyrlily inside the card,phlegm and blood stasis resistance,closed all the empirical,disease with phlegm as the core,the type of disease who also give priority to liver,kidney,spleen,heart;The main disease of the liver in the case of the liver in the case of syndromes is also very important to the mind.Phlegm is the primary disease of refractory epilepsy,and the onset period involves dynamic wind and closed syndrome,the period of interseizure involves the Yin and blood deficiency.3.Through the clinical epidemiological investigation of TCM syndrome type classification and analysis,faint flapping,eye,eye eye stay,dizziness,forgetfulness,god for intractable epilepsy,the main clinical syndrome,faint flapping,mesh on the eyes,convulsions,acute and trismus,the stiffness is the main wind syndrome differentiation syndrome;The main accumulation of the heart,liver,and kidney is the analysis of the syndrome.The pathoid is mainly to move wind,close,phlegm.4.Proteomics study on the intractable epilepsy found that,the expression of MCP-1 in Wind-phlegm blocking orifices,liver stagnation and phlegm heat and blood stasis of is significantly higher than deficiency of both heart and spleen and spleen-kidney deficiency,MCP-1 May as the basic protein of the intractable epilepsy of the empirical TCM,and related to the TCM syndrome elements "the wind","phlegm retention" and "blood stasis".5.Refractory epilepsy related differences in proteomics research found that the expression of P-gp of qi stagnation blood stasis is the most significant,deficiency of bothheart and spleen and spleen-kidney deficiency of P-gp is greater than the Wind-phlegm blocking orifices and liver stagnation and phlegm heat,prompt P-gp or can be used as traditional Chinese medicine "blood stasis" and "insufficiency of the spleen" syndrome common intrinsic signature protein,also prompted Chinese medicine "blood stasis" and "insufficiency of the spleen" may be have the common nature of the protein.