Effect Of Shaoyao Gancao Decoction On The Imbalance Of Th17/Treg And Their Related Cytokine In Rats With Asthma

Objective To observe the effects of Shaoyao Gancao Decoction on the imbalance of Th17/Treg and their related cytokine in rats with asthma,and to explore its mechanism in treating asthma teriostasisMethods Male SD rats were randomly divided into normal control group,model group,Shaoyao Gancao Decoction group,dexamethasone group and integrated tcm-wm group(n=8 for each group).Asthma was induced by intraperitoneal injection of ovalbumin(OVA)and forced inhalation of atomized OVA.BALF cells in each group were collected.Expression of IL-2?IL-6?IL-10 and IL-17 was detected by Elisa method.Numbers of CD4+T cells and Th17 cells in spleen was analyzed by flow cytometry.Results Compared with model group,the total number of cells and the number of eosinophils(Eos)in BALF were decreased significantly in the treated groups(P<0.01).Compared with model group,expression of IL-6,IL-17 and IL-2 in lung homogenate were decreased significantly in the treated group(P<0.01)and expressions of IL-10 was significantly increased in the treated groups(P<0.01);There were no significant differences of expression of IL-2,IL-6 and IL-10 among the treated groups(P>0.05).Expression of IL-17 was decreased significantly in the dexamethasone group than other treated groups(P=0.049?0.006).Compared with model group,Shaoyao Gancao Decoction increased the percentages of CD4+CD25+Foxp3+ regulatory T cell(P<0.01)and decreased the percentages of of Th17 cells(P<0.01).Conclusion Shaoyao Gancao Decoction can efficiently regulates the imbalance of Th17/Treg and their related cytokine,which may be one of its mechanisms in preventing and treating asthma.