The Study On The Development Of Xianlu Huogu Wan And The Mechanism Of The Trestment On ANFH

[PURPOSE] To develope a new creative drug to treat ANFH (Avascular Necrisis of the Femoral Head) named Xianlu Huogu Wan, and to study the treatment mechanism of Xianlu Huogu Wan on ANFH.[METHOD] In this study, we made a curative prescription of ANFH named Xianlu Huogu Wan. The prescription has some innovations on treatment of ANFH by tonifying kidney and healthy bone, promoting blood to dissolve the stasis, promotion and activating collaterals to relieve pain. We broke the insects drugs to worm powder drugs, extracted herbal for the active ingredients to concentrated dose and mix into water-paste pill. we used two types of animal model in rats, retinoic acid-caused and corticosteroid-caused type, and studied the activate function on osteoblast and reduction of osteoblast apoptosis on the cellular level.[RESULT] Xianlu Huogu Wan can improve reduction of the bone mineral density effectively after the lack of blood of femoral head due to retinoic acid induced and enhance the level of bone mineral density of the femoral head and femoral fossa. It can improve the cartilage surface of depression and reduce the degrees of cell degeneration and necrosis of cartilage. It can improve symptoms significantly, such as weight loss of the ANFH rats injected prednisolone acetate, improve blood flow targets, increase the HOM/HOP ratio and improve the bone necrosis caused by hormone by cell flow cytometry and electron microscopy. We isolate and culture the rat osteoblasts (OB), MTT colorimetric cents experiments show that it can increase the generation capabilities of OB and enhanced ALP expression, it can promote OB from G0/G1 phase to S phase, thus contributing to OB mitosis to promote osteoblast proliferation. Genetic tests show that Xianlu Huogu Wan can inhibit the decreased expression of Bcl-2 and increase expression of Bax in the process of OB apoptosis, thereby inhibit the OB necrosis to treat ANFH. The acute toxicity and long-term toxicity test results show that it has low toxicity to be used in clinical practice.[CONCLUSION] As a new medicine of low cost and low side effect, Xianlu Huogu Wan can be used to treat ANFH for long-term. Due to the advanced preparation, good therapeutic effect on hormone-caused and retinoic acid-caused ANFH on rat and the effective protection of osteoblast function in treatment of ANFH, it is worthy to popularize and apply.