Experimental Research On Qilian Shupi Granule Effecting The Activity Of Telomerase、Length Of Telomere And Content Of DNA And Expression Of NF-κB

Objective:To probe into the pharmacodynamic mechanism of Qilian Shupi granule by way of researching on its effecting the activity of telomerase, length of telomere and content of DNA, expression of NF-κB in Wistar rats.Methods:In experiments, models of precancerous lesions of gastric cancer were induced by cancerogenic agent in Wistar rats, which were divided randomly into six groups (normal group, model group, WeiFuchun group and big, middle and small dose QLSP group) according to their weights. After treated with QLSP and WeiFuchun for 12 weeks, main outcome measures:optical microscope was used to observe gastric mucous membrane; Immunohistochemical methods and TRAP-ELISA were used to monitor the expressions of activity of telomerase、length of telomere and expressions of NF-κB, the content of DNA was detected by FCM.Results:Animal experimental research showed that the happened number of gastric mucosal inflammation and atrophy, intestinal metaplasia, hyperplasia of gastric mucous membrane in built models groups was more than normal group, there were notable difference between them (P<0.01 or P<0.05); the length of telomere were increased in big and middle doses QLSP group, while they were increased slightly in Wei Fuchun group and lower greatly in model group, there were notable differences among them(P<0.01); The activity of telomerase were decreased in big and middle doses QLSP group, while they were decreased slightly in Wei Fuchun group and higher greatly in model group, there were notable differences among them(P<0.01). The content of DNA was decreased in big and middle doses QLSP group, while they were higher greatly in model group and decreased slightly in Wei Fuchun group, there were notable differences among them(P< 0.01). The expressions of NF-κB was inhibited in big doses QLSP group, while they were increased greatly in model group and inhibited slightly in Wei Fuchun group, there were notable differences among them (P<0.01).Conclusions:Splenogastric asthenia, accumulation of stagnant pathogenic factors in the body, and involvement of the kidney functions at the advanced stage are the main causes leading to the pathogenesis of precancerous lesions of chronic atrophic gastritis. Therefore, invigorating spleen and tonifying kidney, regulating qi to disperse stagnation, and dispersing accumulation of pathogenic factors to resolve masses are the principles in the management of the condition. The index from experimental research reveals its mechanism at the systemic, cellular and molecular levels in treating precancerous lesions of chronic atrophic gastritis. The QLSP recipe acts on many chains and targets of the disease to produce a comprehensive therapeutic effect, the therapeutic mechanism may have much to do with an overall effect of inhibition of expression of the activity of telomerase、length of telomere and content of DNA、expression of NF-κB. And thus the study enriched the knowledge of pathology and treatment of precancerous lesions of chronic atrophic gastritis.