| Exogenous disease is due to invasion of exogenous pathogens, evil strong and Righteousness weak lead to a class of organs dysfunction Syndrome. Comparison of acute onset of the disease most, often accompanied by fever since the beginning of clinical manifestations, more contagious, epidemic, and seasonal and other features. Influenza and foot and mouth disease is a common seasonal, infectious diseases.The disease caused by viral infection, with rapid onset, rapid development features. The clinical disease with fever chills, general malaise, as main performance.According to both pathogenesis and clinical features, which can be classified as Chinese exogenous disease areas. The huangjin fang are based on clinical experience in medicine, pharmacology research with the development of modern traditional Chinese Herbs.It consists of Huangqin and Jinlianhua two heat drugs commonly used flavor composition. Jun medicine Huangqin with the effect heat dampness and purging fire. Chen medicine Jinlianhua, can help the power of Huangqin Qingrejiedu. Compatibility between the two played a total of detoxification effect. This comprehensive study on the prescription of Huangjin from the extraction process, safety, pharmacology and other aspects, trying to provide accurate experimental basis for treatment of the Influenza and the hand, foot and mouth disease and other exogenous disease.In the extraction study, we used HPLC method, single herb Huangqin extract, Jinlianhua single herb extract, Compound extract gold side three methods of huangjin fang extraction side compound extract from the main active ingredients baicalin, orientin determine the content of glycosides. By comparing the extraction of single herbs together with the compound extracted from extracts of two methods of baicalin, orientin HPLC, preferably better extraction process prescription drugs. The results showed that:a single herb and compound extracted from a common extraction method extracts both baicalin and similar orientin HPLC, combined with industrial production conditions and costs, to determine the extraction of 60% ethanol 3 times, each time a Hours, solid-liquid ratio of 1:10 on the huangjin fang drug compound extracted as crude extract production side the better technology.In drug safety studies, acute toxicity tests in mice, we (the maximum amount of test administration) observe the acute toxicity of the Huangjinfang, experimental results show that:ICR mice by the huangjinfang of the maximum dose volume 172.8g/kg (converted by crude drug),28.8 times of the dose of subsequent experiments in mice pharmacodynamics 6g/kg, the dose of no significant toxicity, safety is high.In pharmacodynamic studies, we have the huangjinfang in vivo antiviral, antibacterial effect in vivo, immune regulation, anti-inflammatory, antipyretic effect a comprehensive evaluation, including the following:1 Use intranasally infected mice with normal mice and the establishment of immunocompromised pneumonia model of influenza virus infection. Treatment and preventive administration were used to observe the Huangjinfang of mouse lung index, mortality, survival time, evaluation of the Huangjin fang strain influenza H1N1 FM1 and PR8 infected rats. The results showed that the Huangjinfang of the normal immune status and inflammation in the lungs of immunocompromised mice a clear role in treatment and prevention can reduce mortality and prolong survival time, the fight against influenza A H1N1 effective drugs.2 Using strains of influenza A H1N1 virus infection in normal mice FM1 pneumonia model, further study of the Huangjinfang of the FM1 strain of influenza virus infection in viral load in lung tissue in mice, IFN-?levels, and immune function, discussing huangjin fang of anti-influenza virus and its mechanism. The results show that the huangjin fang be significantly reduced influenza virus FM1 strains of mice infected lung tissue viral load, improve lung IFN-?levels, regulate serum CD4+, CD8+ cells, the CD4+/CD8+ ratio became normal, and its improve the mechanism of lung inflammation may be by regulating the immune function, inhibit FM1 strain of influenza virus in mouse lung tissue to achieve replication.3 EV71 infection by intraperitoneal injection of suckling mice to observe the Huangjinfang of suckling mice infected with the virus after the general condition, mortality, survival time, evaluation of the Huangjinfang of the treatment of EV71 infection. The results show that Huangjinfang can reduce the EV71 infection the number of neonatal deaths and prolong the average survival time of rats caused by EV71 infection have a protective effect of death.4 Using the cytopathic effect method (CPE) to observed the inhibitory effect of Huangjinfang of influenza virus in vitro, a variety of other common respiratory viruses, EV71 virus inhibition. The results show that the Huangjinfang in vitro PR8 influenza virus strain, brisbane/59/2007 strains of influenza B virus strains in Jiangxi Xiushui, HSV-1, Ad7 and other respiratory viruses that cause a certain degree of cytopathic Inhibition of influenza A H1N1 strains of influenza virus FM1, PIV-1, RSV, Ad3, EV71 no significant inhibition.5 By examining the Huangjin fang of the strains of influenza A H1N1 virus brisbane/59/2007 neuraminidase(NA) activity, to further explore the Huangjin fang mechanism of anti-influenza virus. Experimental results show that the Huangjinfang to inhibit influenza neuraminidase activity trend, but with the virus control group, no significant difference in the inhibitory effect of strains of influenza virus brisban/59 mechanism may be related to reduced neuraminidase activity.6 By studying the Huangjinfang of Staphylococcus aureus death in mice, the Huangjinfang of the antibacterial effect in the body. The results show that Huangjinfang can reduce Staphylococcus aureus in mice after infection mortality, prolonged survival time of mice infected with Staphylococcus aureus has a significant protective effect of death.7 Choose a variety of respiratory infections,intestinal infections and conditions of the common pathogenic bacteria, antibacterial experimental study by the Huangjinfang of the antibacterial effect. The results show that the Huangjinfang in vitro against Staphylococcus aureus, Staphylococcus albus, Staphylococcus epidermidis, beta-hemolytic streptococcus, Streptococcus pneumoniae, the card he cocci, Pseudomonas aeruginosa have different degrees of inhibition. Escherichia coli, Klebsiella pneumoniae, Candida albicans growth in vitro no significant inhibition.8 In this study, mouse ear swelling induced by xylene, acetic acid abdominal capillary permeability of hyperthyroidism, carrageenan induced rat paw swelling in several different animal models of acute inflammation, inflammation of the huangjin fang effects are discussed. The results showed that the Huangjinfang of the mouse ear swelling caused by xylene, acetic acid induced abdominal capillary permeability, carrageenin induced foot swelling had some inhibitory effect, inhibit the early inflammatory telangiectasia exudation induced increase in tissue swelling, with a significant anti-inflammatory effect.9 Using dry yeast induced fever model of endotoxin induced fever in rabbits model, to evaluate the antipyretic effect of the Huangjin fang, experimental results show that the Huangjin fang of the dry yeast induced fever model, model of endotoxin induced fever in rabbits all have significant antipyretic effect.Above results confirm the safety of oral high the Huangjinfang, with in vivo anti-viral, antibacterial, immune regulation, anti-inflammatory, antipyretic effect. The pharmacological effects of exogenous disease for the etiology, pathology and clinical features and other aspects, as the representative of the exogenous influenza Syndrome has significant therapeutic effect. |
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